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PMID: 3020376 Published · ppublish English Journal Article

Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli.

Molecular & general genetics : MGG ·Vol. 204 ·No. 3 ·1986-09-00 ·Pages 367-73

Yamagishi J, Yoshida H, Yamayoshi M, Nakamura S

Abstract

DNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the first base of the 426th codon of the gyrB gene, resulting in an amino acid change from aspartic acid to asparagine, and nal-31 was an A to G transition at the first base of the 447th codon, resulting in an amino acid change from lysine to glutamic acid. This indicates tha mutations in the gyrB gene are responsible for nalidixic acid resistance.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA Topoisomerases, Type II/genetics Drug Resistance, Microbial Escherichia coli/drug effects,enzymology,genetics Genes Genes, Bacterial Mutation Nalidixic Acid/pharmacology Plasmids
Chemicals
Nalidixic Acid DNA Restriction Enzymes DNA Topoisomerases, Type II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yamagishi J
Yoshida H
Yamayoshi M
Nakamura S
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00331012
Published
1986-09-00
Pages
367-73
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
X00870, X04341
External Links
PubMed source DOI: 10.1007/BF00331012
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