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PMID: 6271730 Published · ppublish English Journal Article

New nalidixic acid resistance mutations related to deoxyribonucleic acid gyrase activity.

Journal of bacteriology ·Vol. 148 ·No. 2 ·1981-11-00 ·Pages 450-8

Yamagishi J, Furutani Y, Inoue S, Ohue T, Nakamura S, Shimizu M

Abstract

In Escherichia coli K-12 mutants which had a new nalidixic acid resistance mutation at about 82 min on the chromosome map, cell growth was resistant to or hypersusceptible to nalidixic acid, oxolinic acid, piromidic acid, pipemidic acid, and novobiocin. Deoxyribonucleic acid gyrase activity as tested by supercoiling of lambda phage deoxyribonucleic acid inside the mutants was similarly resistant or hypersusceptible to the compounds. The drug concentrations required for gyrase inhibition were much higher than those for cell growth inhibition but similar to those for inhibition of lambda phage multiplication. Transduction analysis with lambda phages carrying the chromosomal fragment of the tnaA-gyrB region suggested that one of the mutations, nal-31, was located on the gyrB gene.

MeSH Terms
Bacteriophage lambda/growth & development DNA Topoisomerases, Type II/metabolism Drug Resistance, Microbial Escherichia coli/drug effects,genetics Genes, Bacterial Mutation Nalidixic Acid/pharmacology Transduction, Genetic
Chemicals
Nalidixic Acid DNA Topoisomerases, Type II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Yamagishi J
Furutani Y
Inoue S
Ohue T
Nakamura S
Shimizu M
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45 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.148.2.450-458.1981
Published
1981-11-00
Pages
450-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216226
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.148.2.450-458.1981 PMC Full Text View journal details
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