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PMID: 6282812 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bacillus subtilis DNA gyrase: purification of subunits and reconstitution of supercoiling activity.

Journal of bacteriology ·Vol. 151 ·No. 1 ·1982-07-00 ·Pages 524-7

Orr E, Staudenbauer WL

Abstract

DNA gyrase from Bacillus subtilis 168 was purified by affinity chromatography on novobiocin-Sepharose and shown to consist of two subunits, A and B, with molecular weights of 100,000 and 85,000, respectively. The B subunits, which contains novobiocin-sensitive. ATPase activity, could complement the gyrA protein of Escherichia coli. No complementation was detected between the A subunit and the E. coli gyrB protein.

MeSH Terms
Bacillus subtilis/enzymology,genetics DNA Topoisomerases, Type II/isolation & purification,metabolism DNA, Superhelical Genetic Complementation Test Kinetics Macromolecular Substances Molecular Weight
Chemicals
DNA, Superhelical Macromolecular Substances DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Orr E
Staudenbauer W L
References (12)
12 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  3. Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein.
    Anal Biochem. 1978 May;86(1):142-6 PMID: 655375
  4. Micrococcus luteus DNA gyrase: active components and a model for its supercoiling of DNA.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2098-102 PMID: 276855
  5. DNA gyrase and the supercoiling of DNA.
    Science. 1980 Feb 29;207(4434):953-60 PMID: 6243420
  6. Isolation and characterisation of a strain carrying a conditional lethal mutation in the cou gene of Escherichia coli K12.
    Mol Gen Genet. 1979;177(1):103-12 PMID: 94144
  7. Bacillus subtilis deoxyribonucleic acid gyrase.
    J Bacteriol. 1980 Mar;141(3):1331-9 PMID: 6245067
  8. Deoxyribonucleic acid gyrase-deoxyribonucleic acid complex containing 140 base pairs of deoxyribonucleic acid and an alpha 2 beta 2 protein core.
    Biochemistry. 1980 Nov 11;19(23):5229-34 PMID: 6255987
  9. An Escherichia coli mutant thermosensitive in the B subunit of DNA gyrase: effect on the structure and replication of the colicin E1 plasmid in vitro.
    Mol Gen Genet. 1981;181(1):52-6 PMID: 6261085
  10. Replication of phi X174 dna with purified enzymes. I. Conversion of viral DNA to a supercoiled, biologically active duplex.
    J Biol Chem. 1981 May 25;256(10):5233-8 PMID: 6262323
  11. DNA topoisomerases.
    Annu Rev Biochem. 1981;50:879-910 PMID: 6267993
  12. DNA gyrase: affinity chromatography on novobiocin-Sepharose and catalytic properties.
    Nucleic Acids Res. 1981 Aug 11;9(15):3589-603 PMID: 6269086
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.1.524-527.1982
Published
1982-07-00
Pages
524-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220277
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.151.1.524-527.1982 PMC Full Text View journal details
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