Home LiteratureArticle Details
PMID: 2817852 Published · ppublish English Journal Article

Cloning and expression of the norA gene for fluoroquinolone resistance in Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 33 ·No. 9 ·1989-09-00 ·Pages 1535-9

Ubukata K, Itoh-Yamashita N, Konno M

Abstract

The norA gene for fluoroquinolone resistance in Staphylococcus aureus TK2566 was cloned from chromosomal DNA into fluoroquinolone-susceptible Escherichia coli TG1. The resulting transformant, NY11, contained a recombinant plasmid, designated pTUS1, with a cloned 5.5-kilobase (kb) HindIII fragment of staphylococcal DNA. The MIC of norfloxacin for the strain increased from 0.1 to 3.13 micrograms/ml. Furthermore, when the fragment was recloned into S. aureus, the transformant NY12, containing recombinant plasmid pTUS20, had the same level of resistance to norfloxacin as did the original strain, although it was less resistant to ofloxacin and ciprofloxacin. A single KpnI-HaeIII fragment was found to be the minimum size able to express norfloxacin resistance, suggesting that the norA gene is located within the 2.6- to 3.2-kb region of the original 5.5-kb fragment. The 5.5-kb fragment hybridized to DNA from a fluoroquinolone-susceptible S. aureus strain.

MeSH Terms
Anti-Infective Agents/pharmacology Blotting, Southern Chromosome Mapping Cloning, Molecular DNA, Bacterial/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Norfloxacin/pharmacology Plasmids Staphylococcus aureus/drug effects,genetics,ultrastructure Transformation, Genetic
Chemicals
Anti-Infective Agents DNA, Bacterial Norfloxacin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ubukata K
Department of Clinical Pathology, Teikyo University School of Medicine, Tokyo, Japan.
Itoh-Yamashita N
Konno M
References (33)
33 references, click to expand
  1. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies.
    J Bacteriol. 1969 Jul;99(1):238-41 PMID: 4895844
  2. Expression and inducibility in Staphylococcus aureus of the mecA gene, which encodes a methicillin-resistant S. aureus-specific penicillin-binding protein.
    J Bacteriol. 1989 May;171(5):2882-5 PMID: 2708325
  3. DNA gyrase: an enzyme that introduces superhelical turns into DNA.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3872-6 PMID: 186775
  4. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767-71 PMID: 200930
  5. Nalidixic acid resistance: a second genetic character involved in DNA gyrase activity.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4772-6 PMID: 337300
  6. Purification of subunits of Escherichia coli DNA gyrase and reconstitution of enzymatic activity.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1773-7 PMID: 347446
  7. DNA gyrase: subunit structure and ATPase activity of the purified enzyme.
    Proc Natl Acad Sci U S A. 1978 Dec;75(12):5960-3 PMID: 153529
  8. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  9. DNA gyrase: purification and catalytic properties of a fragment of gyrase B protein.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6289-93 PMID: 230505
  10. Gel electrophoresis of restriction fragments.
    Methods Enzymol. 1979;68:152-76 PMID: 232210
  11. In vitro antibacterial activity of AM-715, a new nalidixic acid analog.
    Antimicrob Agents Chemother. 1980 Feb;17(2):103-8 PMID: 6446258
  12. In vitro and in vivo activity of DL-8280, a new oxazine derivative.
    Antimicrob Agents Chemother. 1982 Oct;22(4):548-53 PMID: 6960805
  13. In vitro activity of Bay 09867, a new quinoline derivative, compared with those of other antimicrobial agents.
    Antimicrob Agents Chemother. 1983 Apr;23(4):559-64 PMID: 6222695
  14. Ciprofloxacin, a quinolone carboxylic acid compound active against aerobic and anaerobic bacteria.
    Antimicrob Agents Chemother. 1984 Mar;25(3):319-26 PMID: 6232895
  15. Cloning and simplified purification of Escherichia coli DNA gyrase A and B proteins.
    J Biol Chem. 1984 Jul 25;259(14):9199-201 PMID: 6086626
  16. Genetic and physical organization of the cloned gyrA and gyrB genes of Bacillus subtilis.
    J Bacteriol. 1985 Apr;162(1):78-84 PMID: 2984185
  17. Activity of ciprofloxacin against methicillin-resistant Staphylococcus aureus.
    Antimicrob Agents Chemother. 1985 May;27(5):688-91 PMID: 3160301
  18. Occurrence of a beta-lactam-inducible penicillin-binding protein in methicillin-resistant staphylococci.
    Antimicrob Agents Chemother. 1985 May;27(5):851-7 PMID: 3848294
  19. Mutation affecting resistance of Escherichia coli K12 to nalidixic acid.
    J Gen Microbiol. 1985 Sep;131(9):2285-92 PMID: 3906038
  20. Genetic and biochemical characterization of norfloxacin resistance in Escherichia coli.
    Antimicrob Agents Chemother. 1986 Apr;29(4):639-44 PMID: 3010850
  21. Differences in susceptibility to quinolones of outer membrane mutants of Salmonella typhimurium and Escherichia coli.
    Antimicrob Agents Chemother. 1986 Mar;29(3):535-8 PMID: 3521490
  22. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.
    J Bacteriol. 1986 Sep;167(3):975-80 PMID: 3638304
  23. Nalidixic acid-resistant mutations of the gyrB gene of Escherichia coli.
    Mol Gen Genet. 1986 Sep;204(3):367-73 PMID: 3020376
  24. Isolation and characterization of norfloxacin-resistant mutants of Escherichia coli K-12.
    Antimicrob Agents Chemother. 1986 Aug;30(2):248-53 PMID: 3532944
  25. Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12.
    Eur J Biochem. 1986 Oct 15;160(2):231-8 PMID: 3533535
  26. Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.
    Antimicrob Agents Chemother. 1986 Nov;30(5):777-80 PMID: 3026239
  27. Mutations producing resistance to norfloxacin in Pseudomonas aeruginosa.
    Antimicrob Agents Chemother. 1987 Apr;31(4):582-6 PMID: 3111356
  28. Norfloxacin resistance in a clinical isolate of Escherichia coli.
    Antimicrob Agents Chemother. 1987 Oct;31(10):1640-1 PMID: 2829712
  29. Quinolone-resistant mutations of the gyrA gene of Escherichia coli.
    Mol Gen Genet. 1988 Jan;211(1):1-7 PMID: 2830458
  30. In vitro and in vivo antibacterial activities of T-3262, a new fluoroquinolone.
    Antimicrob Agents Chemother. 1988 Jun;32(6):827-33 PMID: 2843082
  31. Clinical isolate of Citrobacter freundii highly resistant to new quinolones.
    Antimicrob Agents Chemother. 1988 Jun;32(6):922-4 PMID: 2843087
  32. [Trends of fluoroquinolone-resistant strains isolated from clinical materials].
    Kansenshogaku Zasshi. 1988 Jul;62(7):641-51 PMID: 3141525
  33. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.33.9.1535
Published
1989-09-00
Pages
1535-9
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC172697
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.33.9.1535 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2