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PMID: 3026239 Published · ppublish English Journal Article

Purification and properties of DNA gyrase from a fluoroquinolone-resistant strain of Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 30 ·No. 5 ·1986-11-00 ·Pages 777-80

Sato K, Inoue Y, Fujii T, Aoyama H, Inoue M, Mitsuhashi S

Abstract

Subunit A and B proteins of DNA gyrase were separately purified from fluoroquinolone-resistant Escherichia coli GN14181 (MIC of ofloxacin, 100 micrograms/ml) and susceptible strain KL-16. The supercoiling activities of reconstituted Ar+Br (r, resistant) and Ar+Bs (s, susceptible) were 250-fold more resistant to new fluoroquinolones than those of As+Bs and As+Br.

MeSH Terms
Anti-Bacterial Agents/pharmacology DNA Topoisomerases, Type II/isolation & purification Drug Resistance, Microbial Escherichia coli/drug effects,enzymology Hydrocarbons, Fluorinated/pharmacology
Chemicals
Anti-Bacterial Agents Hydrocarbons, Fluorinated DNA Topoisomerases, Type II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sato K
Inoue Y
Fujii T
Aoyama H
Inoue M
Mitsuhashi S
References (20)
20 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.30.5.777
Published
1986-11-00
Pages
777-80
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC176532
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.30.5.777 PMC Full Text View journal details
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