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PMID: 15514046 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

A chromosome bin map of 16,000 expressed sequence tag loci and distribution of genes among the three genomes of polyploid wheat.

Genetics ·Vol. 168 ·No. 2 ·2004-10-00 ·Pages 701-12

Qi LL, Echalier B, Chao S, Lazo GR, Butler GE, Anderson OD, Akhunov ED, Dvorák J, Linkiewicz AM, Ratnasiri A, Dubcovsky J, Bermudez-Kandianis CE, Greene RA, Kantety R, La Rota CM, Munkvold JD, Sorrells SF, Sorrells ME, Dilbirligi M, Sidhu D, Erayman M, Randhawa HS, Sandhu D, Bondareva SN, Gill KS, Mahmoud AA, Ma XF, Miftahudin, Gustafson JP, Conley EJ, Nduati V, Gonzalez-Hernandez JL, Anderson JA, Peng JH, Lapitan NL, Hossain KG, Kalavacharla V, Kianian SF, Pathan MS, Zhang DS, Nguyen HT, Choi DW, Fenton RD, Close TJ, McGuire PE, Qualset CO, Gill BS

Abstract

Because of the huge size of the common wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) genome of 17,300 Mb, sequencing and mapping of the expressed portion is a logical first step for gene discovery. Here we report mapping of 7104 expressed sequence tag (EST) unigenes by Southern hybridization into a chromosome bin map using a set of wheat aneuploids and deletion stocks. Each EST detected a mean of 4.8 restriction fragments and 2.8 loci. More loci were mapped in the B genome (5774) than in the A (5173) or D (5146) genomes. The EST density was significantly higher for the D genome than for the A or B. In general, EST density increased relative to the physical distance from the centromere. The majority of EST-dense regions are in the distal parts of chromosomes. Most of the agronomically important genes are located in EST-dense regions. The chromosome bin map of ESTs is a unique resource for SNP analysis, comparative mapping, structural and functional analysis, and polyploid evolution, as well as providing a framework for constructing a sequence-ready, BAC-contig map of the wheat genome.

MeSH Terms
Chromosome Mapping Chromosomes, Plant/genetics Expressed Sequence Tags Genes, Plant Genetic Markers Genome, Plant Ploidies Quantitative Trait Loci Sequence Alignment Triticum/genetics
Chemicals
Genetic Markers
Authors & Affiliations
47 authors, click to expand affiliations / ORCID
Qi L L
Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, Manhattan, Kansas 66506-5502, USA.
Echalier B
Chao S
Lazo G R
Butler G E
Anderson O D
Akhunov E D
Dvorák J
Linkiewicz A M
Ratnasiri A
Dubcovsky J
Bermudez-Kandianis C E
Greene R A
Kantety R
La Rota C M
Munkvold J D
Sorrells S F
Sorrells M E
Dilbirligi M
Sidhu D
Erayman M
Randhawa H S
Sandhu D
Bondareva S N
Gill K S
Mahmoud A A
Ma X-F
Miftahudin
Gustafson J P
Conley E J
Nduati V
Gonzalez-Hernandez J L
Anderson J A
Peng J H
Lapitan N L V
Hossain K G
Kalavacharla V
Kianian S F
Pathan M S
Zhang D S
Nguyen H T
Choi D-W
Fenton R D
Close T J
McGuire P E
Qualset C O
Gill B S
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.104.034868
Published
2004-10-00
Pages
701-12
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1448828
Subset
IM
External Links
PubMed source DOI: 10.1534/genetics.104.034868 PMC Full Text View journal details
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