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PMID: 15516587 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Responses of the Rhodobacter sphaeroides transcriptome to blue light under semiaerobic conditions.

Journal of bacteriology ·Vol. 186 ·No. 22 ·2004-11-00 ·Pages 7726-35

Braatsch S, Moskvin OV, Klug G, Gomelsky M

Abstract

Exposure to blue light of the facultative phototrophic proteobacterium Rhodobacter sphaeroides grown semiaerobically results in repression of the puc and puf operons involved in photosystem formation. To reveal the genome-wide effects of blue light on gene expression and the underlying photosensory mechanisms, transcriptome profiles of R. sphaeroides during blue-light irradiation (for 5 to 135 min) were analyzed. Expression of most photosystem genes was repressed upon irradiation. Downregulation of photosystem development may be used to prevent photooxidative damage occurring when the photosystem, oxygen, and high-intensity light are present simultaneously. The photoreceptor of the BLUF-domain family, AppA, which belongs to the AppA-PpsR antirepressor-repressor system, is essential for maintenance of repression upon prolonged irradiation (S. Braatsch et al., Mol. Microbiol. 45:827-836, 2002). Transcriptome data suggest that the onset of repression is also mediated by the AppA-PpsR system, albeit via an apparently different sensory mechanism. Expression of several genes, whose products may participate in photooxidative damage defense, including deoxypyrimidine photolyase, glutathione peroxidase, and quinol oxidoreductases, was increased. Among the genes upregulated were genes encoding two sigma factors: sigmaE and sigma38. The consensus promoter sequences for these sigma factors were predicted in the upstream sequences of numerous upregulated genes, suggesting that coordinated action of sigmaE and sigma38 is responsible for the upregulation. Based on the dynamics of upregulation, the anti-sigmaE factor ChrR or its putative upstream partner is proposed to be the primary sensor. The identified transcriptome responses provided a framework for deciphering blue-light-dependent signal transduction pathways in R. sphaeroides.

MeSH Terms
Bacterial Proteins/genetics,metabolism Gene Expression Profiling Gene Expression Regulation, Bacterial Light Oxygen/pharmacology Photosynthetic Reaction Center Complex Proteins/genetics,metabolism Proteome Rhodobacter sphaeroides/genetics,growth & development,metabolism,physiology Transcription, Genetic
Chemicals
Bacterial Proteins Photosynthetic Reaction Center Complex Proteins Proteome Oxygen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Braatsch Stephan
Institut für Mikrobiologie und Molekularbiologie, Universität Giessen, Giesse, Germany.
Moskvin Oleg V
Klug Gabriele
Gomelsky Mark
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.186.22.7726-7735.2004
Published
2004-11-00
Pages
7726-35
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC524910
Subset
IM
Grants
NCRR NIH HHS · P20 RR015640 · United States
NCRR NIH HHS · P20 RR15640 · United States
External Links
PubMed source DOI: 10.1128/JB.186.22.7726-7735.2004 PMC Full Text View journal details
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