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PMID: 15546668 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Type IV secretion: the Agrobacterium VirB/D4 and related conjugation systems.

Biochimica et biophysica acta ·Vol. 1694 ·No. 1-3 ·2004-11-11 ·Pages 219-34

Christie PJ

Abstract

The translocation of DNA across biological membranes is an essential process for many living organisms. In bacteria, type IV secretion systems (T4SS) are used to deliver DNA as well as protein substrates from donor to target cells. The T4SS are structurally complex machines assembled from a dozen or more membrane proteins in response to environmental signals. In Gram-negative bacteria, the conjugation machines are composed of a cell envelope-spanning secretion channel and an extracellular pilus. These dynamic structures (i) direct formation of stable contacts-the mating junction-between donor and recipient cell membranes, (ii) transmit single-stranded DNA as a nucleoprotein particle, as well as protein substrates, across donor and recipient cell membranes, and (iii) mediate disassembly of the mating junction following substrate transfer. This review summarizes recent progress in our understanding of the mechanistic details of DNA trafficking with a focus on the paradigmatic Agrobacterium tumefaciens VirB/D4 T4SS and related conjugation systems.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism,physiology Biological Transport/physiology Conjugation, Genetic/physiology DNA, Bacterial/metabolism Fimbriae, Bacterial/physiology Membrane Transport Proteins/genetics,physiology Molecular Sequence Data Operon Rhizobium/genetics,physiology Virulence Factors/physiology
Chemicals
Bacterial Proteins DNA, Bacterial Membrane Transport Proteins Virulence Factors
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Christie Peter J
Department of Microbiology and Molecular Genetics, UT-Houston Medical School, 6431 Fannin, JFB1.765, Houston TX 77030, USA. [email protected] <[email protected]>
References (111)
111 references, click to expand
  1. Dimerization of the Agrobacterium tumefaciens VirB4 ATPase and the effect of ATP-binding cassette mutations on the assembly and function of the T-DNA transporter.
    Mol Microbiol. 1999 Jun;32(6):1239-53 PMID: 10383764
  2. Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation.
    Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):8173-7 PMID: 10393967
  3. Conjugative pili of IncP plasmids, and the Ti plasmid T pilus are composed of cyclic subunits.
    J Biol Chem. 1999 Aug 6;274(32):22548-55 PMID: 10428832
  4. Bacterial conjugation protein MobA mediates integration of complex DNA structures into plant cells.
    J Bacteriol. 1999 Sep;181(18):5758-65 PMID: 10482518
  5. Analysis of F factor TraD membrane topology by use of gene fusions and trypsin-sensitive insertions.
    J Bacteriol. 1999 Oct;181(19):6108-13 PMID: 10498725
  6. Characterization of ATP and DNA binding activities of TrwB, the coupling protein essential in plasmid R388 conjugation.
    J Biol Chem. 1999 Dec 17;274(51):36117-24 PMID: 10593894
  7. Vir proteins stabilize VirB5 and mediate its association with the T pilus of Agrobacterium tumefaciens.
    J Bacteriol. 1999 Dec;181(24):7485-92 PMID: 10601205
  8. The Agrobacterium T-DNA transport pore proteins VirB8, VirB9, and VirB10 interact with one another.
    J Bacteriol. 2000 Feb;182(3):758-63 PMID: 10633111
  9. TraG from RP4 and TraG and VirD4 from Ti plasmids confer relaxosome specificity to the conjugal transfer system of pTiC58.
    J Bacteriol. 2000 Mar;182(6):1541-8 PMID: 10692358
  10. Components of the RP4 conjugative transfer apparatus form an envelope structure bridging inner and outer membranes of donor cells: implications for related macromolecule transport systems.
    J Bacteriol. 2000 Mar;182(6):1564-74 PMID: 10692361
  11. Sequence-related protein export NTPases encoded by the conjugative transfer region of RP4 and by the cag pathogenicity island of Helicobacter pylori share similar hexameric ring structures.
    Proc Natl Acad Sci U S A. 2000 Mar 28;97(7):3067-72 PMID: 10716714
  12. Conjugative junctions in RP4-mediated mating of Escherichia coli.
    J Bacteriol. 2000 May;182(10):2709-15 PMID: 10781537
  13. A new ABC transporter mediating the detachment of lipid-modified proteins from membranes.
    Nat Cell Biol. 2000 Apr;2(4):212-8 PMID: 10783239
  14. Genetic and environmental factors affecting T-pilin export and T-pilus biogenesis in relation to flagellation of Agrobacterium tumefaciens.
    J Bacteriol. 2000 Jul;182(13):3705-16 PMID: 10850985
  15. The N- and C-terminal portions of the Agrobacterium VirB1 protein independently enhance tumorigenesis.
    J Bacteriol. 2000 Jun;182(12):3437-45 PMID: 10852875
  16. The bases of crown gall tumorigenesis.
    J Bacteriol. 2000 Jul;182(14):3885-95 PMID: 10869063
  17. VirB6 is required for stabilization of VirB5 and VirB3 and formation of VirB7 homodimers in Agrobacterium tumefaciens.
    J Bacteriol. 2000 Aug;182(16):4505-11 PMID: 10913084
  18. Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells.
    Trends Microbiol. 2000 Aug;8(8):354-60 PMID: 10920394
  19. Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: an evolving story.
    Microbes Infect. 2000 Jul;2(9):1061-72 PMID: 10967286
  20. Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob.
    Mol Microbiol. 2000 Sep;37(6):1283-92 PMID: 10998162
  21. VirB/D4-dependent protein translocation from Agrobacterium into plant cells.
    Science. 2000 Nov 3;290(5493):979-82 PMID: 11062129
  22. DNA-independent transport of plasmid primase protein between bacteria by the I1 conjugation system.
    Mol Microbiol. 2000 Nov;38(3):650-7 PMID: 11069687
  23. Maturation of IncP pilin precursors resembles the catalytic Dyad-like mechanism of leader peptidases.
    J Bacteriol. 2000 Dec;182(23):6751-61 PMID: 11073921
  24. Crystal structure of the hexameric traffic ATPase of the Helicobacter pylori type IV secretion system.
    Mol Cell. 2000 Dec;6(6):1461-72 PMID: 11163218
  25. The bacterial conjugation protein TrwB resembles ring helicases and F1-ATPase.
    Nature. 2001 Feb 1;409(6820):637-41 PMID: 11214325
  26. Phylogeny of genes for secretion NTPases: identification of the widespread tadA subfamily and development of a diagnostic key for gene classification.
    Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2503-8 PMID: 11226268
  27. Functional analysis of the Agrobacterium tumefaciens T-DNA transport pore protein VirB8.
    J Bacteriol. 2001 Jun;183(12):3636-41 PMID: 11371528
  28. VirB7 lipoprotein is exocellular and associates with the Agrobacterium tumefaciens T pilus.
    J Bacteriol. 2001 Jun;183(12):3642-51 PMID: 11371529
  29. RpoS co-operates with other factors to induce Legionella pneumophila virulence in the stationary phase.
    Mol Microbiol. 2001 Jun;40(5):1201-14 PMID: 11401723
  30. DNA transport in bacteria.
    Nat Rev Mol Cell Biol. 2001 Jul;2(7):538-45 PMID: 11433368
  31. A variable genetic island specific for Neisseria gonorrhoeae is involved in providing DNA for natural transformation and is found more often in disseminated infection isolates.
    Mol Microbiol. 2001 Jul;41(1):263-77 PMID: 11454218
  32. Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system.
    Mol Microbiol. 2001 Jul;41(2):379-91 PMID: 11489125
  33. Bacterial gliding motility: multiple mechanisms for cell movement over surfaces.
    Annu Rev Microbiol. 2001;55:49-75 PMID: 11544349
  34. Role of Agrobacterium VirB11 ATPase in T-pilus assembly and substrate selection.
    J Bacteriol. 2001 Oct;183(20):5813-25 PMID: 11566978
  35. Nonspecific adherence and fibril biogenesis by Actinobacillus actinomycetemcomitans: TadA protein is an ATPase.
    J Bacteriol. 2001 Oct;183(20):5927-36 PMID: 11566992
  36. The carboxy-terminus of VirE2 from Agrobacterium tumefaciens is required for its transport to host cells by the virB-encoded type IV transport system.
    Mol Microbiol. 2001 Sep;41(6):1283-93 PMID: 11580834
  37. Cellular location and temperature-dependent assembly of IncHI1 plasmid R27-encoded TrhC-associated conjugative transfer protein complexes.
    Mol Microbiol. 2001 Nov;42(3):705-15 PMID: 11722736
  38. Evidence that F-plasmid proteins TraV, TraK and TraB assemble into an envelope-spanning structure in Escherichia coli.
    Mol Microbiol. 2001 Nov;42(3):757-66 PMID: 11722740
  39. Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions.
    Microbiology. 2001 Dec;147(Pt 12):3201-14 PMID: 11739753
  40. Biogenesis of T pili in Agrobacterium tumefaciens requires precise VirB2 propilin cleavage and cyclization.
    J Bacteriol. 2002 Jan;184(1):327-30 PMID: 11741876
  41. Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein. Detailed structural features and mapping of the active site cleft.
    J Biol Chem. 2002 Mar 1;277(9):7556-66 PMID: 11748238
  42. The Brucella suis virB operon is induced intracellularly in macrophages.
    Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1544-9 PMID: 11830669
  43. TrwD, the hexameric traffic ATPase encoded by plasmid R388, induces membrane destabilization and hemifusion of lipid vesicles.
    J Bacteriol. 2002 Mar;184(6):1661-8 PMID: 11872717
  44. Bacterial secrets of secretion: EuroConference on the biology of type IV secretion processes.
    Mol Microbiol. 2002 Mar;43(5):1359-65 PMID: 11918819
  45. Functional complexity of the twin-arginine translocase TatC component revealed by site-directed mutagenesis.
    Mol Microbiol. 2002 Mar;43(6):1457-70 PMID: 11952898
  46. Polar location and functional domains of the Agrobacterium tumefaciens DNA transfer protein VirD4.
    Mol Microbiol. 2002 Mar;43(6):1523-32 PMID: 11952902
  47. TraG-like proteins of DNA transfer systems and of the Helicobacter pylori type IV secretion system: inner membrane gate for exported substrates?
    J Bacteriol. 2002 May;184(10):2767-79 PMID: 11976307
  48. The PtlE protein of Bordetella pertussis has peptidoglycanase activity required for Ptl-mediated pertussis toxin secretion.
    J Bacteriol. 2002 Jun;184(11):2863-9 PMID: 12003925
  49. Structure and role of coupling proteins in conjugal DNA transfer.
    Res Microbiol. 2002 May;153(4):199-204 PMID: 12066890
  50. Bacterial conjugation: a two-step mechanism for DNA transport.
    Mol Microbiol. 2002 Jul;45(1):1-8 PMID: 12100543
  51. Peptide linkage mapping of the Agrobacterium tumefaciens vir-encoded type IV secretion system reveals protein subassemblies.
    Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11493-500 PMID: 12177441
  52. Detergent extraction identifies different VirB protein subassemblies of the type IV secretion machinery in the membranes of Agrobacterium tumefaciens.
    Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11405-10 PMID: 12177443
  53. Agrobacterium type IV secretion is a two-step process in which export substrates associate with the virulence protein VirJ in the periplasm.
    Mol Microbiol. 2002 Sep;45(5):1325-35 PMID: 12207700
  54. Purification and properties of TrwB, a hexameric, ATP-binding integral membrane protein essential for R388 plasmid conjugation.
    J Biol Chem. 2002 Nov 29;277(48):46456-62 PMID: 12244053
  55. Aquifex aeolicus PilT, homologue of a surface motility protein, is a thermostable oligomeric NTPase.
    J Bacteriol. 2002 Dec;184(23):6465-71 PMID: 12426333
  56. AAA proteins.
    Curr Opin Struct Biol. 2002 Dec;12(6):746-53 PMID: 12504679
  57. Non-permanent proteins in membranes: when proteins come as visitors (Review).
    Mol Membr Biol. 2002 Oct-Dec;19(4):237-45 PMID: 12512770
  58. The VirB4 family of proposed traffic nucleoside triphosphatases: common motifs in plasmid RP4 TrbE are essential for conjugation and phage adsorption.
    J Bacteriol. 2003 Feb;185(3):1045-58 PMID: 12533481
  59. Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3.
    Nucleic Acids Res. 2003 Feb 1;31(3):860-8 PMID: 12560481
  60. Production of the type IV secretion system differs among Brucella species as revealed with VirB5- and VirB8-specific antisera.
    Infect Immun. 2003 Mar;71(3):1075-82 PMID: 12595417
  61. Type IV transporters of pathogenic bacteria.
    Curr Opin Microbiol. 2003 Feb;6(1):29-34 PMID: 12615216
  62. Agrobacterium tumefaciens VirB6 protein participates in formation of VirB7 and VirB9 complexes required for type IV secretion.
    J Bacteriol. 2003 May;185(9):2867-78 PMID: 12700266
  63. VirB11 ATPases are dynamic hexameric assemblies: new insights into bacterial type IV secretion.
    EMBO J. 2003 May 1;22(9):1969-80 PMID: 12727865
  64. Functional subsets of the virB type IV transport complex proteins involved in the capacity of Agrobacterium tumefaciens to serve as a recipient in virB-mediated conjugal transfer of plasmid RSF1010.
    J Bacteriol. 2003 Jun;185(11):3259-69 PMID: 12754223
  65. Polar localization of replicon origins in the multipartite genomes of Agrobacterium tumefaciens and Sinorhizobium meliloti.
    J Bacteriol. 2003 Jun;185(11):3384-91 PMID: 12754237
  66. Show me the substrates: modulation of host cell function by type IV secretion systems.
    Cell Microbiol. 2003 Jun;5(6):373-83 PMID: 12780775
  67. Conjugative plasmid transfer in gram-positive bacteria.
    Microbiol Mol Biol Rev. 2003 Jun;67(2):277-301, table of contents PMID: 12794193
  68. Interaction between the IncHI1 plasmid R27 coupling protein and type IV secretion system: TraG associates with the coiled-coil mating pair formation protein TrhB.
    Mol Microbiol. 2003 Jul;49(1):105-16 PMID: 12823814
  69. F factor conjugation is a true type IV secretion system.
    FEMS Microbiol Lett. 2003 Jul 15;224(1):1-15 PMID: 12855161
  70. TraG-like proteins of type IV secretion systems: functional dissection of the multiple activities of TraG (RP4) and TrwB (R388).
    J Bacteriol. 2003 Aug;185(15):4371-81 PMID: 12867445
  71. VirE2, a type IV secretion substrate, interacts with the VirD4 transfer protein at cell poles of Agrobacterium tumefaciens.
    Mol Microbiol. 2003 Sep;49(6):1699-713 PMID: 12950931
  72. Recognition of the Agrobacterium tumefaciens VirE2 translocation signal by the VirB/D4 transport system does not require VirE1.
    Plant Physiol. 2003 Nov;133(3):978-88 PMID: 14551327
  73. Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae.
    J Mol Biol. 2003 Oct 24;333(3):657-74 PMID: 14556751
  74. The outs and ins of bacterial type IV secretion substrates.
    Trends Microbiol. 2003 Nov;11(11):527-35 PMID: 14607070
  75. Structural and functional characterization of the VirB5 protein from the type IV secretion system encoded by the conjugative plasmid pKM101.
    Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15947-52 PMID: 14673074
  76. Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer.
    Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):841-6 PMID: 14715899
  77. The Legionella pneumophila PilT homologue DotB exhibits ATPase activity that is critical for intracellular growth.
    J Bacteriol. 2004 Mar;186(6):1658-66 PMID: 14996796
  78. The versatile bacterial type IV secretion systems.
    Nat Rev Microbiol. 2003 Nov;1(2):137-49 PMID: 15035043
  79. The VirD2 protein of A. tumefaciens contains a C-terminal bipartite nuclear localization signal: implications for nuclear uptake of DNA in plant cells.
    Cell. 1992 Jan 10;68(1):109-18 PMID: 1732061
  80. Conjugational junctions: morphology of specific contacts in conjugating Escherichia coli bacteria.
    J Struct Biol. 1991 Oct;107(2):146-56 PMID: 1807350
  81. Protein transfer into the recipient cell during bacterial conjugation: studies with F and RP4.
    Mol Microbiol. 1990 Jul;4(7):1199-205 PMID: 2172695
  82. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  83. Gene 19 of plasmid R1 is required for both efficient conjugative DNA transfer and bacteriophage R17 infection.
    J Bacteriol. 1995 Aug;177(15):4279-88 PMID: 7543471
  84. The octopine-type Ti plasmid pTiA6 of Agrobacterium tumefaciens contains a gene homologous to the chromosomal virulence gene acvB.
    J Bacteriol. 1995 Feb;177(4):892-7 PMID: 7860597
  85. Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable.
    J Bacteriol. 1994 Jul;176(14):4455-8 PMID: 8021231
  86. Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes.
    J Bacteriol. 1994 Jun;176(12):3646-60 PMID: 8206843
  87. Subcellular localization of seven VirB proteins of Agrobacterium tumefaciens: implications for the formation of a T-DNA transport structure.
    J Bacteriol. 1993 Aug;175(16):5233-41 PMID: 8349563
  88. The Agrobacterium tumefaciens virB4 gene product is an essential virulence protein requiring an intact nucleoside triphosphate-binding domain.
    J Bacteriol. 1993 Mar;175(6):1723-34 PMID: 8449880
  89. The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface.
    J Bacteriol. 1996 Jun;178(11):3156-67 PMID: 8655494
  90. The Agrobacterium tumefaciens VirB7 lipoprotein is required for stabilization of VirB proteins during assembly of the T-complex transport apparatus.
    J Bacteriol. 1996 Jun;178(11):3168-76 PMID: 8655495
  91. Intermolecular disulfide bonds stabilize VirB7 homodimers and VirB7/VirB9 heterodimers during biogenesis of the Agrobacterium tumefaciens T-complex transport apparatus.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):7512-7 PMID: 8755505
  92. Resection and mutagenesis of the acid pH-inducible P2 promoter of the Agrobacterium tumefaciens virG gene.
    J Bacteriol. 1996 Aug;178(15):4717-20 PMID: 8755905
  93. Enzymology of DNA transfer by conjugative mechanisms.
    Prog Nucleic Acid Res Mol Biol. 1996;54:197-251 PMID: 8768076
  94. Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein complex.
    Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8889-94 PMID: 8799123
  95. Interactions between VirB9 and VirB10 membrane proteins involved in movement of DNA from Agrobacterium tumefaciens into plant cells.
    J Bacteriol. 1997 Jan;179(1):78-89 PMID: 8981983
  96. The VirB4 ATPase of Agrobacterium tumefaciens is a cytoplasmic membrane protein exposed at the periplasmic surface.
    J Bacteriol. 1997 Jan;179(2):453-62 PMID: 8990298
  97. Characterization of membrane and protein interaction determinants of the Agrobacterium tumefaciens VirB11 ATPase.
    J Bacteriol. 1997 Feb;179(3):583-91 PMID: 9006008
  98. The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens.
    J Bacteriol. 1997 Feb;179(4):1211-8 PMID: 9023204
  99. Agrobacterium tumefaciens T-complex transport apparatus: a paradigm for a new family of multifunctional transporters in eubacteria.
    J Bacteriol. 1997 May;179(10):3085-94 PMID: 9150199
  100. Delineation of the interaction domains of Agrobacterium tumefaciens VirB7 and VirB9 by use of the yeast two-hybrid assay.
    J Bacteriol. 1997 Jun;179(11):3404-9 PMID: 9171381
  101. Genetic evidence of a coupling role for the TraG protein family in bacterial conjugation.
    Mol Gen Genet. 1997 Apr 28;254(4):400-6 PMID: 9180693
  102. Suppression of mutant phenotypes of the Agrobacterium tumefaciens VirB11 ATPase by overproduction of VirB proteins.
    J Bacteriol. 1997 Sep;179(18):5835-42 PMID: 9294442
  103. The cytoplasmic DNA-binding protein TraM binds to the inner membrane protein TraD in vitro.
    J Bacteriol. 1997 Oct;179(19):6133-7 PMID: 9324263
  104. Nicking by transesterification: the reaction catalysed by a relaxase.
    Mol Microbiol. 1997 Sep;25(6):1011-22 PMID: 9350859
  105. The filamentous phage pIV multimer visualized by scanning transmission electron microscopy.
    Science. 1997 Nov 28;278(5343):1635-8 PMID: 9374466
  106. The outer membrane component, YscC, of the Yop secretion machinery of Yersinia enterocolitica forms a ring-shaped multimeric complex.
    Mol Microbiol. 1997 Nov;26(4):789-97 PMID: 9427408
  107. Construction of transposon Tn3phoA: its application in defining the membrane topology of the Agrobacterium tumefaciens DNA transfer proteins.
    Mol Microbiol. 1998 Jan;27(2):405-14 PMID: 9484895
  108. The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane.
    Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3146-51 PMID: 9501230
  109. The Ti plasmid increases the efficiency of Agrobacterium tumefaciens as a recipient in virB-mediated conjugal transfer of an IncQ plasmid.
    Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):7057-62 PMID: 9618538
  110. The AAA team: related ATPases with diverse functions.
    Trends Cell Biol. 1998 Feb;8(2):65-71 PMID: 9695811
  111. The carboxyl terminus of protein TraD adds specificity and efficiency to F-plasmid conjugative transfer.
    J Bacteriol. 1998 Nov;180(22):6039-42 PMID: 9811665
Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbamcr.2004.02.013
Email
[email protected]
Published
2004-11-11
Pages
219-34
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC4845649
Subset
IM
Grants
NIGMS NIH HHS · R01 GM048746 · United States
NIGMS NIH HHS · GM47846 · United States
External Links
PubMed source DOI: 10.1016/j.bbamcr.2004.02.013 PMC Full Text
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