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PMID: 15548595 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Xrs2p regulates Mre11p translocation to the nucleus and plays a role in telomere elongation and meiotic recombination.

Molecular biology of the cell ·Vol. 16 ·No. 2 ·2005-02-00 ·Pages 597-608

Tsukamoto Y, Mitsuoka C, Terasawa M, Ogawa H, Ogawa T

Abstract

The Mre11-Rad50-Xrs2 (MRX) protein complex plays pivotal roles in meiotic recombination, repair of damaged DNA, telomere elongation, and cell cycle checkpoint control. Xrs2p is known to be essential for all the functions of the complex, but its role in the complex has not been clearly elucidated. A 32-amino acid region near the C terminus of Xrs2p was identified as an Mre11p-binding site. No more function of Xrs2p than translocation of Mre11p from the cytoplasm to the nucleus is necessary for response to DNA damage. However, domains in Xrs2p located both 49 amino acids upstream and 104 amino acids downstream of the Mre11p binding site are required for meiotic recombination and telomere elongation, respectively, in addition to the 32-amino acid region. These findings demonstrate that Xrs2p acts as a specificity factor that allows the MRX complex to function in meiotic recombination and in telomere elongation.

MeSH Terms
Alleles Amino Acid Sequence Binding Sites Cell Nucleus/metabolism Cytoplasm/metabolism DNA Repair Endodeoxyribonucleases/metabolism Exodeoxyribonucleases/metabolism Immunohistochemistry Meiosis Point Mutation Precipitin Tests Protein Binding Protein Structure, Tertiary Recombination, Genetic Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism Telomere/metabolism Translocation, Genetic Two-Hybrid System Techniques
Chemicals
Saccharomyces cerevisiae Proteins XRS2 protein, S cerevisiae Endodeoxyribonucleases Exodeoxyribonucleases MRE11 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Tsukamoto Yasumasa
Iwate College of Nursing, Iwate 020-0151, Japan. [email protected]
Mitsuoka Chikako
Terasawa Masahiro
Ogawa Hideyuki
Ogawa Tomoko
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-09-0782
Email
[email protected]
Published
2005-02-00
Epub
2004-00-17
Pages
597-608
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC545897
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e04-09-0782 PMC Full Text View journal details
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