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PMID: 15549137 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.

The EMBO journal ·Vol. 23 ·No. 24 ·2004-12-08 ·Pages 4868-75

Aylon Y, Liefshitz B, Kupiec M

Abstract

DNA double-strand breaks (DSBs) are dangerous lesions that can lead to genomic instability and cell death. Eukaryotic cells repair DSBs either by nonhomologous end-joining (NHEJ) or by homologous recombination. We investigated the ability of yeast cells (Saccharomyces cerevisiae) to repair a single, chromosomal DSB by recombination at different stages of the cell cycle. We show that cells arrested at the G1 phase of the cell cycle restrict homologous recombination, but are able to repair the DSB by NHEJ. Furthermore, we demonstrate that recombination ability does not require duplicated chromatids or passage through S phase, and is controlled at the resection step by Clb-CDK activity.

MeSH Terms

Animals Cell Cycle/physiology Cyclin-Dependent Kinases/genetics,metabolism DNA Damage DNA Repair Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism

Chemicals

Saccharomyces cerevisiae Proteins Cyclin-Dependent Kinases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Aylon Yael

Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.

Liefshitz Batia

Kupiec Martin

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25 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

DOI

10.1038/sj.emboj.7600469

Published

2004-12-08

Epub

2004-00-18

Pages

4868-75

Language

English

Region

England

NLM ID

8208664

PMCID

PMC535085

Subset

External Links

PubMed source DOI: 10.1038/sj.emboj.7600469 PMC Full Text View journal details

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The CDK regulates repair of double-strand breaks by homologous recombination during the cell cycle.

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