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PMID: 12944484 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The checkpoint protein Rad24 of Saccharomyces cerevisiae is involved in processing double-strand break ends and in recombination partner choice.

Molecular and cellular biology ·Vol. 23 ·No. 18 ·2003-09-00 ·Pages 6585-96

Aylon Y, Kupiec M

Abstract

Upon chromosomal damage, cells activate a checkpoint response that includes cell cycle arrest and a stimulation of DNA repair. The checkpoint protein Rad24 is key to the survival of a single, repairable double-strand break (DSB). However, the low survival of rad24 cells is not due to their inability to arrest cell cycle progression. In rad24 mutants, processing of the broken ends is delayed and protracted, resulting in extended kinetics of DSB repair and in cell death. The limited resection of rad24 mutants also affects recombination partner choice by a mechanism dependent on the length of the interacting homologous donor sequences. Unexpectedly, rad24 cells with a DSB eventually accumulate and die at the G(2)/M phase of the cell cycle. This arrest depends on the spindle checkpoint protein Mad2.

MeSH Terms
Calcium-Binding Proteins/genetics,metabolism Carrier Proteins Cell Cycle/genetics Cell Cycle Proteins/genetics,metabolism DNA/genetics,metabolism DNA Repair/physiology Fungal Proteins/genetics,metabolism Genetic Techniques Intracellular Signaling Peptides and Proteins Kinetics Mad2 Proteins Mutation Nuclear Proteins Recombination, Genetic/physiology Saccharomyces cerevisiae/cytology,genetics Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Calcium-Binding Proteins Carrier Proteins Cell Cycle Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins MAD2 protein, S cerevisiae Mad2 Proteins Nuclear Proteins RAD24 protein, S cerevisiae Saccharomyces cerevisiae Proteins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Aylon Yael
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv 69978, Israel.
Kupiec Martin
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.18.6585-6596.2003
Published
2003-09-00
Pages
6585-96
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC193701
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.18.6585-6596.2003 PMC Full Text View journal details
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