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PMID: 10825202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA repair protein Rad55 is a terminal substrate of the DNA damage checkpoints.

Molecular and cellular biology ·Vol. 20 ·No. 12 ·2000-06-00 ·Pages 4393-404

Bashkirov VI, King JS, Bashkirova EV, Schmuckli-Maurer J, Heyer WD

Abstract

Checkpoints, which are integral to the cellular response to DNA damage, coordinate transient cell cycle arrest and the induced expression of DNA repair genes after genotoxic stress. DNA repair ensures cellular survival and genomic stability, utilizing a multipathway network. Here we report evidence that the two systems, DNA damage checkpoint control and DNA repair, are directly connected by demonstrating that the Rad55 double-strand break repair protein of the recombinational repair pathway is a terminal substrate of DNA damage and replication block checkpoints. Rad55p was specifically phosphorylated in response to DNA damage induced by the alkylating agent methyl methanesulfonate, dependent on an active DNA damage checkpoint. Rad55p modification was also observed after gamma ray and UV radiation. The rapid time course of phosphorylation and the recombination defects identified in checkpoint-deficient cells are consistent with a role of the DNA damage checkpoint in activating recombinational repair. Rad55p phosphorylation possibly affects the balance between different competing DNA repair pathways.

MeSH Terms
DNA Damage/radiation effects DNA Repair DNA, Fungal DNA-Binding Proteins Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Phosphorylation Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Ultraviolet Rays
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins RAD55 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bashkirov V I
Institute of General Microbiology, CH-3012 Bern, Switzerland.
King J S
Bashkirova E V
Schmuckli-Maurer J
Heyer W D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.20.12.4393-4404.2000
Published
2000-06-00
Pages
4393-404
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC85806
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.20.12.4393-4404.2000 PMC Full Text View journal details
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