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PMID: 1556157 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study.

The Journal of cell biology ·Vol. 117 ·No. 1 ·1992-04-00 ·Pages 73-82

Kolodney MS, Wysolmerski RB

Abstract

We have used an isometric force transducer to study contraction of two types of nonmuscle cells in tissue culture. This method permits the quantitative measurement of contractile force generated by cells of defined type under the influence of external agents while allowing detailed morphological observation. Chick embryo fibroblasts (CEF), which form a contractile network inside a collagen matrix, and human umbilical vein endothelial cells (HUVE), which are located in a monolayer on the surface of the collagen matrix, were studied. CEF and HUVE in 10% FCS produce a substantial tension of 4.5 +/- 0.2 x 10(4) dynes/cm2 and 6.1 x 10(4) dynes/cm2, respectively. Both cell types contract when stimulated with thrombin, generating a force per cell cross-sectional area of approximately 10(5) dynes/cm2, a value approximately an order of magnitude less than smooth muscle. The integrity of the actin cytoskeleton is essential for force generation, as disruption of actin microfilaments with cytochalasin D results in a rapid disappearance of force. Intact microtubules appear to reduce isometric force exerted by CEF, as microtubule-disrupting drugs result in increased tension. Contraction by HUVE precedes a dramatic rearrangement of actin microfilaments from a circumferential ring to stress fibers.

MeSH Terms
Actins/analysis Animals Cell Membrane/physiology,ultrastructure Cells, Cultured Chick Embryo Collagen Endothelium, Vascular/drug effects,physiology Fibroblasts/drug effects,physiology Fluorescent Antibody Technique Humans Kinetics Muscle Contraction Thrombin/pharmacology Time Factors
Chemicals
Actins Collagen Thrombin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kolodney M S
Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 63110.
Wysolmerski R B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
Published
1992-04-00
Pages
73-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289400
Subset
IM
Grants
NIGMS NIH HHS · GM-38838 · United States
NHLBI NIH HHS · HL-30572 · United States
NHLBI NIH HHS · HL-45788 · United States
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