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PMID: 155688 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Arrangement of the genes coding for ribosomal ribonucleic acids in Neurospora crassa.

Journal of bacteriology ·Vol. 137 ·No. 3 ·1979-03-00 ·Pages 1219-26

Free SJ, Rice PW, Metzenberg RL

Abstract

We have cloned and characterized Neurospora crassa ribosomal deoxyribonucleic acid (rDNA). The rDNA is found as a tandemly repeated 6.0-megadalton sequence. We have mapped a portion of the rDNA repeat unit with respect to its sites for 13 restriction endonucleases and defined those regions coding for the 5. 8S, 17S, and 26S ribosomal ribonucleic acids (rRNA's). We have also isolated several clones containing 5S rRNA sequences. The 5S rRNA coding sequences are not found within the rDNA repeat unit. We found that the sequences surrounding the 5S rRNA coding regions are highly heterogeneous.

MeSH Terms
DNA Restriction Enzymes/metabolism DNA, Recombinant/analysis Escherichia coli/genetics Genes Genetic Code Neurospora/genetics Neurospora crassa/analysis,genetics Nucleic Acid Hybridization RNA, Ribosomal/genetics Transformation, Genetic
Chemicals
DNA, Recombinant RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Free S J
Rice P W
Metzenberg R L
References (25)
25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.137.3.1219-1226.1979
Published
1979-03-00
Pages
1219-26
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC218304
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.137.3.1219-1226.1979 PMC Full Text View journal details
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