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PMID: 15574876 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synthetic genetic array analysis of the PtdIns 4-kinase Pik1p identifies components in a Golgi-specific Ypt31/rab-GTPase signaling pathway.

Molecular biology of the cell ·Vol. 16 ·No. 2 ·2005-02-00 ·Pages 776-93

Sciorra VA, Audhya A, Parsons AB, Segev N, Boone C, Emr SD

Abstract

Phosphorylated derivatives of phosphatidylinositol are essential regulators of both endocytic and exocytic trafficking in eukaryotic cells. In Saccharomyces cerevisiae, the phosphatidylinositol 4-kinase, Pik1p generates a distinct pool of PtdIns(4)P that is required for normal Golgi structure and secretory function. Here, we utilize a synthetic genetic array analysis of a conditional pik1 mutant to identify candidate components of the Pik1p/PtdIns(4)P signaling pathway at the Golgi. Our data suggest a mechanistic involvement for Pik1p with a specific subset of Golgi-associated proteins, including the Ypt31p rab-GTPase and the TRAPPII protein complex, to regulate protein trafficking through the secretory pathway. We further demonstrate that TRAPPII specifically functions in a Ypt31p-dependent pathway and identify Gyp2p as the first biologically relevant GTPase activating protein for Ypt31p. We propose that multiple stage-specific signals, which may include Pik1p/PtdIns(4)P, TRAPPII and Gyp2p, impinge upon Ypt31 signaling to regulate Golgi secretory function.

MeSH Terms
1-Phosphatidylinositol 4-Kinase/genetics,metabolism,ultrastructure Blotting, Western Chitin Synthase GTPase-Activating Proteins/metabolism Gene Expression Regulation, Enzymologic Golgi Apparatus/metabolism,ultrastructure Green Fluorescent Proteins/metabolism Membrane Proteins/metabolism Microarray Analysis Microscopy, Confocal Models, Biological Mutation Precipitin Tests Protein Structure, Tertiary Protein Transport R-SNARE Proteins Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Saccharomyces cerevisiae Proteins/genetics,metabolism,ultrastructure Signal Transduction Temperature Vesicular Transport Proteins/metabolism rab GTP-Binding Proteins/chemistry,metabolism
Chemicals
GTPase-Activating Proteins Gyp2 protein, S cerevisiae Membrane Proteins R-SNARE Proteins SNC2 protein, S cerevisiae Saccharomyces cerevisiae Proteins TRS130 protein, S cerevisiae TRS65 protein, S cerevisiae Vesicular Transport Proteins transport protein particle, TRAPP Green Fluorescent Proteins CHS3 protein, S cerevisiae Chitin Synthase 1-Phosphatidylinositol 4-Kinase PIK1 protein, S cerevisiae rab GTP-Binding Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sciorra Vicki A
Department of Cellular and Molecular Medicine, Howard Hughes Medical Institute, School of Medicine, University of California at San Diego, La Jolla, CA 92093-0668, USA.
Audhya Anjon
Parsons Ainslie B
Segev Nava
Boone Charles
Emr Scott D
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-08-0700
Published
2005-02-00
Epub
2004-00-01
Pages
776-93
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC545911
Subset
IM
Grants
NCI NIH HHS · CA58689 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-08-0700 PMC Full Text View journal details
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