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PMID: 11689439 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes.

The EMBO journal ·Vol. 20 ·No. 21 ·2001-11-01 ·Pages 5991-8

Siniossoglou S, Pelham HR

Abstract

Membrane traffic requires vesicles to fuse with a specific target, and SNARE proteins and Rab/Ypt GTPases contribute to this specificity. In the yeast Saccharomyces cerevisae, the Rab/Ypt GTPase Ypt6p is required for fusion of endosome-derived vesicles with the late Golgi. We have shown previously that activation of Ypt6p depends on its exchange factor, Ric1p-Rgp1p, a peripheral membrane protein complex restricted to the Golgi. We show here that a conserved trimeric protein complex, VFT (Vps52/53/54), binds directly to Ypt6p:GTP. Localization of VFT to the Golgi requires Ypt6p, but is unaffected in gos1 and tlg1 mutants, in which late Golgi integral membrane proteins, including SNAREs, are mislocalized. The VFT complex also binds directly to the N-terminal domain of the SNARE Tlg1p, both in vitro and in vivo, in a Ypt6p-independent manner. We suggest that the VFT complex links vesicles containing Tlg1p to their target, which is defined by the local activation of Ypt6p.

MeSH Terms
Cytoplasmic Vesicles/metabolism Endosomes/metabolism Fungal Proteins/metabolism Golgi Apparatus/metabolism Intracellular Membranes/metabolism Macromolecular Substances Membrane Fusion/physiology Membrane Proteins/metabolism Monomeric GTP-Binding Proteins/genetics,metabolism Protein Binding/physiology Protein Structure, Tertiary/physiology Recombinant Fusion Proteins/genetics,metabolism SNARE Proteins Saccharomyces cerevisiae Proteins/metabolism Vesicular Transport Proteins
Chemicals
Fungal Proteins Macromolecular Substances Membrane Proteins Recombinant Fusion Proteins SNARE Proteins Saccharomyces cerevisiae Proteins VPS52 protein, S cerevisiae VPS54 protein, S cerevisiae Vesicular Transport Proteins Monomeric GTP-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Siniossoglou S
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Pelham H R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/20.21.5991
Published
2001-11-01
Pages
5991-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125711
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/20.21.5991 PMC Full Text View journal details
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