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PMID: 10189369 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Golgi structure correlates with transitional endoplasmic reticulum organization in Pichia pastoris and Saccharomyces cerevisiae.

The Journal of cell biology ·Vol. 145 ·No. 1 ·1999-04-05 ·Pages 69-81

Rossanese OW, Soderholm J, Bevis BJ, Sears IB, O'Connor J, Williamson EK, Glick BS

Abstract

Golgi stacks are often located near sites of "transitional ER" (tER), where COPII transport vesicles are produced. This juxtaposition may indicate that Golgi cisternae form at tER sites. To explore this idea, we examined two budding yeasts: Pichia pastoris, which has coherent Golgi stacks, and Saccharomyces cerevisiae, which has a dispersed Golgi. tER structures in the two yeasts were visualized using fusions between green fluorescent protein and COPII coat proteins. We also determined the localization of Sec12p, an ER membrane protein that initiates the COPII vesicle assembly pathway. In P. pastoris, Golgi stacks are adjacent to discrete tER sites that contain COPII coat proteins as well as Sec12p. This arrangement of the tER-Golgi system is independent of microtubules. In S. cerevisiae, COPII vesicles appear to be present throughout the cytoplasm and Sec12p is distributed throughout the ER, indicating that COPII vesicles bud from the entire ER network. We propose that P. pastoris has discrete tER sites and therefore generates coherent Golgi stacks, whereas S. cerevisiae has a delocalized tER and therefore generates a dispersed Golgi. These findings open the way for a molecular genetic analysis of tER sites.

MeSH Terms
Carrier Proteins/genetics,metabolism Cell Cycle Endoplasmic Reticulum/ultrastructure Fungal Proteins/metabolism Genes, Reporter Golgi Apparatus/ultrastructure Green Fluorescent Proteins Guanine Nucleotide Exchange Factors Intracellular Membranes/ultrastructure Luminescent Proteins/analysis,genetics Membrane Glycoproteins/metabolism Microscopy, Electron Microscopy, Immunoelectron Microtubules/drug effects Nocodazole/pharmacology Phosphoproteins/genetics,metabolism Pichia/ultrastructure Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/ultrastructure Saccharomyces cerevisiae Proteins Species Specificity Vesicular Transport Proteins
Chemicals
Carrier Proteins Fungal Proteins Guanine Nucleotide Exchange Factors Luminescent Proteins Membrane Glycoproteins Phosphoproteins Recombinant Fusion Proteins SEC12 protein, S cerevisiae SEC31 protein, S cerevisiae Saccharomyces cerevisiae Proteins Vesicular Transport Proteins Green Fluorescent Proteins Nocodazole
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rossanese O W
Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, Illinois 60637, USA.
Soderholm J
Bevis B J
Sears I B
O'Connor J
Williamson E K
Glick B S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.145.1.69
Published
1999-04-05
Pages
69-81
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2148216
Subset
IM
Grants
PHS HHS · 5-20942 · United States
External Links
PubMed source DOI: 10.1083/jcb.145.1.69 PMC Full Text View journal details
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