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PMID: 8972206 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mammalian homolog of yeast Sec13p is enriched in the intermediate compartment and is essential for protein transport from the endoplasmic reticulum to the Golgi apparatus.

Molecular and cellular biology ·Vol. 17 ·No. 1 ·1997-01-00 ·Pages 256-66

Tang BL, Peter F, Krijnse-Locker J, Low SH, Griffiths G, Hong W

Abstract

The role of COPII components in endoplasmic reticulum (ER)-Golgi transport, first identified in the yeast Saccharomyces cerevisiae, has yet to be fully characterized in higher eukaryotes. A human cDNA whose predicted amino acid sequence showed 70% similarity to the yeast Sec13p has previously been cloned. Antibodies raised against the human SEC13 protein (mSEC13) recognized a cellular protein of 35 kDa in both the soluble and membrane fractions. Like the yeast Sec13p, mSEC13 exist in the cytosol in both monomeric and higher-molecular-weight forms. Immunofluorescence microscopy localized mSEC13 to the characteristic spotty ER-Golgi intermediate compartment (ERGIC) in cells of all species examined, where it colocalized well with the KDEL receptor, an ERGIC marker, at 15 degrees C. Immunoelectron microscopy also localized mSEC13 to membrane structures close to the Golgi apparatus. mSEC13 is essential for ER-to-Golgi transport, since both the His6-tagged mSEC13 recombinant protein and the affinity-purified mSEC13 antibody inhibited the transport of restrictive temperature-arrested vesicular stomatitis virus G protein from the ER to the Golgi apparatus in a semi-intact cell assay. Moreover, cytosol immunodepleted of mSEC13 could no longer support ER-Golgi transport. Transport could be restored in a dose-dependent manner by a cytosol fraction enriched in the high-molecular-weight mSEC13 complex but not by a fraction enriched in either monomeric mSEC13 or recombinant mSEC13. As a putative component of the mammalian COPII complex, mSEC13 showed partially overlapping but mostly different properties in terms of localization, membrane recruitment, and dynamics compared to that of beta-COP, a component of the COPI complex.

MeSH Terms
Animals Biological Transport Cell Line Coatomer Protein Cytosol/chemistry Endoplasmic Reticulum/chemistry,metabolism Fungal Proteins/analysis,metabolism Golgi Apparatus/chemistry,metabolism Humans Mammals Membrane Glycoproteins Membrane Proteins/analysis,metabolism Microtubule-Associated Proteins/analysis Nuclear Pore Complex Proteins Receptors, Peptide/analysis Recombinant Fusion Proteins/analysis Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Temperature Vesicular stomatitis Indiana virus Viral Envelope Proteins/metabolism
Chemicals
Coatomer Protein Fungal Proteins G protein, vesicular stomatitis virus KDEL receptor Membrane Glycoproteins Membrane Proteins Microtubule-Associated Proteins Nuclear Pore Complex Proteins Receptors, Peptide Recombinant Fusion Proteins SEC13 protein, S cerevisiae Saccharomyces cerevisiae Proteins Viral Envelope Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tang B L
Membrane Biology Laboratory, National University of Singapore, Republic of Singapore.
Peter F
Krijnse-Locker J
Low S H
Griffiths G
Hong W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.1.256
Published
1997-01-00
Pages
256-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231750
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.17.1.256 PMC Full Text View journal details
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