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PMID: 8598201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

GPI anchor attachment is required for Gas1p transport from the endoplasmic reticulum in COP II vesicles.

The EMBO journal ·Vol. 15 ·No. 1 ·1996-01-02 ·Pages 182-91

Doering TL, Schekman R

Abstract

Inositol starvation of auxotrophic yeast interrupts glycolipid biosynthesis and prevents lipid modification of a normally glycosyl phosphatidylinositol (GPI)-linked protein, Gas1p. The unanchored Gas1p precursor undergoes progressive modification in the endoplasmic reticulum (ER), but is not modified by Golgi-specific glycosylation. Starvation-induced defects in anchor assembly and protein processing are rapid, and occur without altered maturation of other proteins. Cells remain competent to manufacture anchor components and to process Gas1p efficiently once inositol is restored. Newly synthesized Gas1p is packaged into vesicles formed in vitro from perforated yeast spheroplasts incubated with either yeast cytosol or the purified Sec proteins (COP II) required for vesicle budding from the ER. In vitro synthesized vesicles produced by inositol-starved membranes do not contain detectable Gas1p. These studies demonstrate that COP II components fulfill the soluble protein requirements for packaging a GPI-anchored protein into ER-derived transport vesicles. However, GPI anchor attachment is required for this packaging to occur.

MeSH Terms
Biological Transport Cell Compartmentation Endoplasmic Reticulum/metabolism Fungal Proteins/metabolism,physiology Glycosylphosphatidylinositols/metabolism Inositol/metabolism Membrane Glycoproteins/metabolism Membrane Proteins/metabolism,physiology Nuclear Pore Complex Proteins Organelles/metabolism Protein Processing, Post-Translational Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins GAS1 protein, S cerevisiae Glycosylphosphatidylinositols Membrane Glycoproteins Membrane Proteins Nuclear Pore Complex Proteins SEC13 protein, S cerevisiae Saccharomyces cerevisiae Proteins Inositol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doering T L
Department of Molecular and Cell Biology, Howard Hughes Research Institute, University of California, Berkeley, 94720, USA.
Schekman R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1996-01-02
Pages
182-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC449930
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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