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PMID: 8070398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ceramide synthesis enhances transport of GPI-anchored proteins to the Golgi apparatus in yeast.

The EMBO journal ·Vol. 13 ·No. 16 ·1994-08-15 ·Pages 3687-95

Horvath A, Sütterlin C, Manning-Krieg U, Movva NR, Riezman H

Abstract

Inhibition of ceramide synthesis by a fungal metabolite, myriocin, leads to a rapid and specific reduction in the rate of transport of glycosylphosphatidylinositol (GPI)-anchored proteins to the Golgi apparatus without affecting transport of soluble or transmembrane proteins. Inhibition of ceramide biosynthesis also quickly blocks remodelling of GPI anchors to their ceramide-containing, mild base-resistant forms. These results suggest that the pool of ceramide is rapidly depleted from early points of the secretory pathway and that its presence at these locations enhances transport of GPI-anchored proteins specifically. A mutant that is resistant to myriocin reverses its effect on GPI-anchored protein transport without reversing its effects on ceramide synthesis and remodelling. Two hypotheses are proposed to explain the role of ceramide in the transport of GPI-anchored proteins.

MeSH Terms
Acyltransferases/antagonists & inhibitors Antifungal Agents/pharmacology Biological Transport/drug effects Ceramides/metabolism Fatty Acids, Monounsaturated/pharmacology Fungal Proteins/metabolism Glycosylphosphatidylinositols/metabolism Golgi Apparatus/metabolism Membrane Glycoproteins/metabolism Mutation Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Serine C-Palmitoyltransferase Sphingolipids/metabolism
Chemicals
Antifungal Agents Ceramides Fatty Acids, Monounsaturated Fungal Proteins GAS1 protein, S cerevisiae Glycosylphosphatidylinositols Membrane Glycoproteins Saccharomyces cerevisiae Proteins Sphingolipids Acyltransferases Serine C-Palmitoyltransferase thermozymocidin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Horvath A
Biozentrum University of Basel, Switzerland.
Sütterlin C
Manning-Krieg U
Movva N R
Riezman H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-08-15
Pages
3687-95
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395279
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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