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PMID: 7730400 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yeast Gaa1p is required for attachment of a completed GPI anchor onto proteins.

The Journal of cell biology ·Vol. 129 ·No. 3 ·1995-05-00 ·Pages 629-39

Hamburger D, Egerton M, Riezman H

Abstract

Anchoring of proteins to membranes by glycosylphosphatidylinositols (GPIs) is ubiquitous among all eukaryotes and heavily used by parasitic protozoa. GPI is synthesized and transferred en bloc to form GPI-anchored proteins. The key enzyme in this process is a putative GPI:protein transamidase that would cleave a peptide bond near the COOH terminus of the protein and attach the GPI by an amide linkage. We have identified a gene, GAA1, encoding an essential ER protein required for GPI anchoring. gaal mutant cells synthesize the complete GPI anchor precursor at nonpermissive temperatures, but do not attach it to proteins. Overexpression of GAA1 improves the ability of cells to attach anchors to a GPI-anchored protein with a mutant anchor attachment site. Therefore, Gaa1p is required for a terminal step of GPI anchor attachment and could be part of the putative GPI:protein transamidase.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Endocytosis Endoplasmic Reticulum/chemistry Genes, Fungal/genetics Glycosylation Glycosylphosphatidylinositols/metabolism Inositol/metabolism Mannose/metabolism Membrane Glycoproteins/biosynthesis,genetics,isolation & purification,metabolism Molecular Sequence Data Mutation Protein Processing, Post-Translational Recombinant Proteins/biosynthesis Saccharomyces cerevisiae Proteins Yeasts/genetics,metabolism
Chemicals
GAA1 protein, S cerevisiae GAS1 protein, S cerevisiae Glycosylphosphatidylinositols Membrane Glycoproteins Recombinant Proteins Saccharomyces cerevisiae Proteins Inositol Mannose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hamburger D
Biozentrum of the University of Basel, Switzerland.
Egerton M
Riezman H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.129.3.629
Published
1995-05-00
Pages
629-39
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120449
Subset
IM
Databases
GENBANK
X79409
External Links
PubMed source DOI: 10.1083/jcb.129.3.629 PMC Full Text View journal details
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