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PMID: 8294512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A phosphatidylinositol transfer protein controls the phosphatidylcholine content of yeast Golgi membranes.

The Journal of cell biology ·Vol. 124 ·No. 3 ·1994-02-00 ·Pages 273-87

McGee TP, Skinner HB, Whitters EA, Henry SA, Bankaitis VA

Abstract

SEC14p is required for protein transport from the yeast Golgi complex. We describe a quantitative analysis of yeast bulk membrane and Golgi membrane phospholipid composition under conditions where Golgi secretory function has been uncoupled from its usual SEC14p requirement. The data demonstrate that SEC14p specifically functions to maintain a reduced phosphatidylcholine content in Golgi membranes and indicate that overproduction of SEC14p markedly reduces the apparent rate of phosphatidylcholine biosynthesis via the CDP-choline pathway in vivo. We suggest that SEC14p serves as a sensor of Golgi membrane phospholipid composition through which the activity of the CDP-choline pathway in Golgi membranes is regulated such that a phosphatidylcholine content that is compatible with the essential secretory function of these membranes is maintained.

MeSH Terms
Carrier Proteins/genetics,metabolism Cytidine Diphosphate Choline/metabolism Golgi Apparatus/chemistry,metabolism Intracellular Membranes/chemistry,metabolism Membrane Proteins Models, Biological Mutation Phosphatidylcholines/analysis,biosynthesis Phosphatidylinositols/metabolism Phospholipid Transfer Proteins Phospholipids/analysis Saccharomyces cerevisiae Proteins Yeasts
Chemicals
Carrier Proteins Membrane Proteins Phosphatidylcholines Phosphatidylinositols Phospholipid Transfer Proteins Phospholipids SEC24 protein, S cerevisiae Saccharomyces cerevisiae Proteins Cytidine Diphosphate Choline
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
McGee T P
Department of Cell Biology, University of Alabama at Birmingham 35294-0005.
Skinner H B
Whitters E A
Henry S A
Bankaitis V A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.124.3.273
Published
1994-02-00
Pages
273-87
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119930
Subset
IM
Grants
NIGMS NIH HHS · GM19629 · United States
NIGMS NIH HHS · GM44530 · United States
External Links
PubMed source DOI: 10.1083/jcb.124.3.273 PMC Full Text View journal details
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