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PMID: 8007981 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A pathway for cell wall anchorage of Saccharomyces cerevisiae alpha-agglutinin.

Molecular and cellular biology ·Vol. 14 ·No. 7 ·1994-07-00 ·Pages 4825-33

Lu CF, Kurjan J, Lipke PN

Abstract

Saccharomyces cerevisiae alpha-agglutinin is a cell wall-anchored adhesion glycoprotein. The previously identified 140-kDa form, which contains a glycosyl-phosphatidylinositol (GPI) anchor (D. Wojciechowicz, C.-F. Lu, J. Kurjan, and P. N. Lipke, Mol. Cell. Biol. 13:2554-2563, 1993), and additional forms of 80, 150, 250 to 300, and > 300 kDa had the properties of intermediates in a transport and cell wall anchorage pathway. N glycosylation and additional modifications resulted in successive increases in size during transport. The 150- and 250- to 300-kDa forms were membrane associated and are likely to be intermediates between the 140-kDa form and a cell surface GPI-anchored form of > 300 kDa. A soluble form of > 300 kDa that lacked the GPI anchor had properties of a periplasmic intermediate between the plasma membrane form and the > 300-kDa cell wall-anchored form. These results constitute experimental support for the hypothesis that GPI anchors act to localize alpha-agglutinin to the plasma membrane and that cell wall anchorage involves release from the GPI anchor to produce a periplasmic intermediate followed by linkage to the cell wall.

MeSH Terms
Cell Wall/physiology Endopeptidase K Glucan Endo-1,3-beta-D-Glucosidase/metabolism Glycosylphosphatidylinositols/metabolism Inositol/metabolism Kinetics Mating Factor Membrane Glycoproteins/biosynthesis,isolation & purification Methionine/metabolism Molecular Weight Palmitic Acid Palmitic Acids/metabolism Peptide Biosynthesis Peptides/isolation & purification Phosphatidylinositol Diacylglycerol-Lyase Phosphoric Diester Hydrolases/metabolism Saccharomyces cerevisiae/genetics,physiology Serine Endopeptidases/metabolism
Chemicals
Glycosylphosphatidylinositols Membrane Glycoproteins Palmitic Acids Peptides Palmitic Acid Inositol Mating Factor Methionine Phosphoric Diester Hydrolases Glucan Endo-1,3-beta-D-Glucosidase Serine Endopeptidases Endopeptidase K Phosphatidylinositol Diacylglycerol-Lyase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lu C F
Department of Biological Sciences, Hunter College, City University of New York, New York 10021.
Kurjan J
Lipke P N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.7.4825-4833.1994
Published
1994-07-00
Pages
4825-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358855
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.7.4825-4833.1994 PMC Full Text View journal details
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