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PMID: 1756718 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Saccharomyces cerevisiae a- and alpha-agglutinin: characterization of their molecular interaction.

The EMBO journal ·Vol. 10 ·No. 13 ·1991-12-00 ·Pages 4081-8

Cappellaro C, Hauser K, Mrśa V, Watzele M, Watzele G, Gruber C, Tanner W

Abstract

An O-glycosylated protein of approximately 18 kDa responsible for mating type specific agglutination has been isolated from Saccharomyces cerevisiae a cells, purified to homogeneity and via peptide sequences the gene was cloned by PCR. An open reading frame codes for a protein of 69 amino acids. A minimum of five serine and five threonine residues of the mature protein are glycosylated. alpha-Agglutinin is a highly N-glycosylated protein of approximately 250 kDa. Both purified agglutinins form a specific 1:1 complex in vitro. Pretreatment of alpha-agglutinin, but not of alpha-agglutinin, with diethylpyrocarbonate (DEPC) prevents formation of the complex; treatment of alpha-agglutinin in the presence of alpha-agglutinin protects the former from DEPC inactivation. By carboxy terminal shortening of the alpha-agglutinin gene and by replacing three of its eight histidyl residues by arginine, the active region of alpha-agglutinin for interaction with alpha-agglutinin has been defined. Neither the N- nor the O-linked saccharides of the two agglutinins seem to be essential for their interaction.

MeSH Terms
Base Sequence Blotting, Northern Cloning, Molecular Diethyl Pyrocarbonate/chemistry Fungal Proteins/genetics,metabolism Glycosylation Mating Factor Molecular Sequence Data Mutagenesis, Site-Directed Open Reading Frames Peptide Mapping Peptides/genetics,metabolism Pheromones/metabolism Polymerase Chain Reaction RNA, Fungal/analysis Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Trypsin
Chemicals
Fungal Proteins Peptides Pheromones RNA, Fungal Saccharomyces cerevisiae Proteins a-agglutinin protein, S cerevisiae Mating Factor Trypsin Diethyl Pyrocarbonate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cappellaro C
Lehrstuhl für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, FRG.
Hauser K
Mrśa V
Watzele M
Watzele G
Gruber C
Tanner W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1991-12-00
Pages
4081-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC453156
Subset
IM
Databases
GENBANK
M62511, M62512, M62513, M62514, M62515, S65080, S65083, S72668, X62877, X62936
External Links
PubMed source PMC Full Text View journal details
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