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PMID: 15591127 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development.

Molecular biology of the cell ·Vol. 16 ·No. 2 ·2005-02-00 ·Pages 689-700

He Y, Fang X, Emoto K, Jan YN, Adler PN

Abstract

The Trc/Ndr/Sax1/Cbk1 family of ser/thr kinases plays a key role in the morphogenesis of polarized cell structures in flies, worms, and yeast. Tricornered (Trc), the Drosophila nuclear Dbf2-related (Ndr) serine/threonine protein kinase, is required for the normal morphogenesis of epidermal hairs, bristles, laterals, and dendrites. We obtained in vivo evidence that Trc function was regulated by phosphorylation and that mutations in key regulatory sites resulted in dominant negative alleles. We found that wild-type, but not mutant Trc, is found in growing hairs, and we failed to detect Trc in pupal wing nuclei, implying that in this developmental context Trc functions in the cytoplasm. The furry gene and its homologues in yeast and Caenorhabditis elegans have previously been implicated as being essential for the function of the Ndr kinase family. We found that Drosophila furry (Fry) also is found in growing hairs, that its subcellular localization is dependent on Trc function, and that it can be coimmunoprecipitated with Trc. Our data suggest a feedback mechanism involving Trc activity regulates the accumulation of Fry in developing hairs.

MeSH Terms
Animals Cells, Cultured Drosophila/cytology,enzymology,genetics,growth & development Drosophila Proteins/genetics,metabolism Gene Expression Regulation, Developmental Genes, Insect Hair/growth & development,metabolism Immunohistochemistry Microscopy, Confocal Models, Biological Mutation Nuclear Proteins/genetics,metabolism Phosphorylation Protein Serine-Threonine Kinases/genetics,metabolism Pupa Signal Transduction Transfection Wings, Animal/growth & development,metabolism
Chemicals
Drosophila Proteins Nuclear Proteins fry protein, Drosophila trc protein, Drosophila Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
He Ying
Department of Biology, Center for Morphogenesis and Regenerative Medicine and Cancer Center, University of Virginia, Charlottesville, VA 22903, USA.
Fang Xiaolan
Emoto Kazuo
Jan Yuh-Nung
Adler Paul N
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e04-09-0828
Published
2005-02-00
Epub
2004-00-09
Pages
689-700
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC545904
Subset
IM
Grants
NIGMS NIH HHS · R01 GM053498 · United States
NINDS NIH HHS · R01 NS040929 · United States
NIGMS NIH HHS · GM-53498 · United States
NINDS NIH HHS · R01NS40929 · United States
External Links
PubMed source DOI: 10.1091/mbc.e04-09-0828 PMC Full Text View journal details
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