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PMID: 7761441 Published · ppublish English Comparative Study Journal Article

Molecular cloning and characterization of a conserved nuclear serine(threonine) protein kinase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 11 ·1995-05-23 ·Pages 5022-6

Millward T, Cron P, Hemmings BA

Abstract

Human, Drosophila melanogaster, and Caenorhabditis elegans cDNA clones encoding homologues of a serine(threonine) protein kinase (EC 2.7.1.37) (designated Ndr protein kinase) have been isolated and sequenced. The human and Drosophila cDNAs predict polypeptides of 54 kDa and 52 kDa, respectively, which share approximately 80% amino acid similarity. Northern analysis of human tissues revealed a ubiquitously expressed 3.9-kb transcript. Recombinant GST-Ndr underwent intramolecular autophosphorylation on serine and threonine residues in vitro but failed to transphosphorylate several standard protein kinase substrates. Transfection of the human cDNA into COS-1 cells resulted in the appearance of an intense nuclear staining in cells analyzed by indirect immunofluorescence; deletion mutagenesis identified a short basic peptide, KRKAETWKRNRR, responsible for the nuclear accumulation of Ndr. Thus, Ndr is a conserved and widely expressed nuclear protein kinase. The closest known relative of this previously uncharacterized kinase is Dbf2, a budding yeast protein kinase required for the completion of nuclear division.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans/enzymology Cell Line Cell Nucleus/enzymology Chlorocebus aethiops Cloning, Molecular Conserved Sequence DNA Primers DNA, Complementary Drosophila/embryology,enzymology Glutathione Transferase/biosynthesis HeLa Cells Humans Kinetics Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/analysis,biosynthesis,chemistry Polymerase Chain Reaction Protein Serine-Threonine Kinases/analysis,biosynthesis,chemistry Recombinant Fusion Proteins/analysis,biosynthesis,chemistry Sequence Deletion Sequence Homology, Amino Acid Transfection
Chemicals
DNA Primers DNA, Complementary Nuclear Proteins Recombinant Fusion Proteins Glutathione Transferase Protein Serine-Threonine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Millward T
Friedrich Miescher-Institut, Basel, Switzerland.
Cron P
Hemmings B A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.11.5022
Published
1995-05-23
Pages
5022-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC41840
Subset
IM
Databases
GENBANK
Z34989, Z35102, Z35103
External Links
PubMed source DOI: 10.1073/pnas.92.11.5022 PMC Full Text View journal details
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