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PMID: 15655113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The control of Spo11's interaction with meiotic recombination hotspots.

Genes & development ·Vol. 19 ·No. 2 ·2005-01-15 ·Pages 255-69

Prieler S, Penkner A, Borde V, Klein F

Abstract

Programmed double-strand breaks (DSBs), which initiate meiotic recombination, arise through the activity of the evolutionary conserved topoisomerase homolog Spo11. Spo11 is believed to catalyze the DNA cleavage reaction in the initial step of DSB formation, while at least a further 11 factors assist in Saccharomyces cerevisiae. Using chromatin-immunoprecipitation (ChIP), we detected the transient, noncovalent association of Spo11 with meiotic hotspots in wild-type cells. The establishment of this association requires Rec102, Rec104, and Rec114, while the timely removal of Spo11 from chromatin depends on several factors, including Mei4 and Ndt80. In addition, at least one further component, namely, Red1, is responsible for locally restricting Spo11's interaction to the core region of the hotspot. In chromosome spreads, we observed meiosis-specific Spo11-Myc foci, independent of DSB formation, from leptotene until pachytene. In both rad50S and com1Delta/sae2Delta mutants, we observed a novel reaction intermediate between Spo11 and hotspots, which leads to the detection of full-length hotspot DNA by ChIP in the absence of artificial cross-linking. Although this DNA does not contain a break, its recovery requires Spo11's catalytic residue Y135. We propose that detection of uncross-linked full-length hotspot DNA is only possible during the reversible stage of the Spo11 cleavage reaction, in which rad50S and com1Delta/sae2Delta mutants transiently arrest.

MeSH Terms
Amino Acid Substitution/genetics Chromatin/genetics,metabolism Chromatin Immunoprecipitation Chromosome Breakage/genetics,physiology Chromosomes, Fungal/genetics,physiology DNA, Fungal/genetics,metabolism Endodeoxyribonucleases Esterases/genetics,metabolism Gene Deletion Gene Expression Regulation, Fungal/genetics,physiology Multiprotein Complexes/genetics,metabolism Pachytene Stage/genetics,physiology Point Mutation/genetics Recombination, Genetic/physiology Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/genetics,metabolism Synaptonemal Complex/genetics,metabolism
Chemicals
Chromatin DNA, Fungal Multiprotein Complexes Saccharomyces cerevisiae Proteins Endodeoxyribonucleases Esterases meiotic recombination protein SPO11
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Prieler Silvia
Institute of Botany, Max F. Perutz Laboratories, Department of Chromosome Biology, A-1030 Vienna, Austria.
Penkner Alexandra
Borde Valérie
Klein Franz
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.321105
Published
2005-01-15
Pages
255-69
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC545890
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.321105 PMC Full Text View journal details
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