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PMID: 15660156 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Major structural differences and novel potential virulence mechanisms from the genomes of multiple campylobacter species.

PLoS biology ·Vol. 3 ·No. 1 ·2005-01-00 ·Pages e15

Fouts DE, Mongodin EF, Mandrell RE, Miller WG, Rasko DA, Ravel J, Brinkac LM, DeBoy RT, Parker CT, Daugherty SC, Dodson RJ, Durkin AS, Madupu R, Sullivan SA, Shetty JU, Ayodeji MA, Shvartsbeyn A, Schatz MC, Badger JH, Fraser CM, Nelson KE

Abstract

Sequencing and comparative genome analysis of four strains of Campylobacter including C. lari RM2100, C. upsaliensis RM3195, and C. coli RM2228 has revealed major structural differences that are associated with the insertion of phage- and plasmid-like genomic islands, as well as major variations in the lipooligosaccharide complex. Poly G tracts are longer, are greater in number, and show greater variability in C. upsaliensis than in the other species. Many genes involved in host colonization, including racR/S, cadF, cdt, ciaB, and flagellin genes, are conserved across the species, but variations that appear to be species specific are evident for a lipooligosaccharide locus, a capsular (extracellular) polysaccharide locus, and a novel Campylobacter putative licABCD virulence locus. The strains also vary in their metabolic profiles, as well as their resistance profiles to a range of antibiotics. It is evident that the newly identified hypothetical and conserved hypothetical proteins, as well as uncharacterized two-component regulatory systems and membrane proteins, may hold additional significant information on the major differences in virulence among the species, as well as the specificity of the strains for particular hosts.

MeSH Terms
Animals Bacterial Proteins/genetics Bird Diseases/microbiology Birds Campylobacter/classification,genetics,pathogenicity Cattle Cattle Diseases/microbiology Genome, Bacterial Likelihood Functions Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Amino Acid Swine Swine Diseases/microbiology Virulence/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
21 authors, click to expand affiliations / ORCID
Fouts Derrick E
The Institute for Genomic Research, Rockville, Maryland, United States of America. [email protected]
Mongodin Emmanuel F
Mandrell Robert E
Miller William G
Rasko David A
Ravel Jacques
Brinkac Lauren M
DeBoy Robert T
Parker Craig T
Daugherty Sean C
Dodson Robert J
Durkin A Scott
Madupu Ramana
Sullivan Steven A
Shetty Jyoti U
Ayodeji Mobolanle A
Shvartsbeyn Alla
Schatz Michael C
Badger Jonathan H
Fraser Claire M
Nelson Karen E
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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.0030015
Email
[email protected]
Published
2005-01-00
Epub
2005-00-04
Pages
e15
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC539331
Subset
IM
Databases
GENBANK
CP000025
External Links
PubMed source DOI: 10.1371/journal.pbio.0030015 PMC Full Text View journal details
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