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PMID: 1566583 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Hemagglutinin activation of pathogenic avian influenza viruses of serotype H7 requires the protease recognition motif R-X-K/R-R.

Virology ·Vol. 188 ·No. 1 ·1992-05-00 ·Pages 408-13

Vey M, Orlich M, Adler S, Klenk HD, Rott R, Garten W

Abstract

The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowl/Victoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens.

MeSH Terms
Amino Acid Sequence Base Sequence DNA, Viral Endopeptidases/metabolism Hemagglutinins, Viral/metabolism Influenza A virus/genetics,metabolism,pathogenicity Molecular Sequence Data Mutagenesis, Site-Directed Proline/metabolism
Chemicals
DNA, Viral Hemagglutinins, Viral Proline Endopeptidases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vey M
Institut für Virologie, Philipps-Universität Marburg, Germany.
Orlich M
Adler S
Klenk H D
Rott R
Garten W
References (36)
36 references, click to expand
  1. Sequence requirements for cleavage activation of influenza virus hemagglutinin expressed in mammalian cells.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):324-8 PMID: 2829180
  2. Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.
    Nature. 1980 Nov 20;288(5788):236-41 PMID: 6985476
  3. Analysis of the relationship between cleavability of a paramyxovirus fusion protein and length of the connecting peptide.
    J Virol. 1989 Mar;63(3):1293-301 PMID: 2644448
  4. Role of Staphylococcus protease in the development of influenza pneumonia.
    Nature. 1987 Feb 5-11;325(6104):536-7 PMID: 3543690
  5. Mutations within the proteolytic cleavage site of the Rous sarcoma virus glycoprotein that block processing to gp85 and gp37.
    J Virol. 1987 May;61(5):1609-14 PMID: 3033286
  6. Intracellular targeting and structural conservation of a prohormone-processing endoprotease.
    Science. 1989 Oct 27;246(4929):482-6 PMID: 2683070
  7. Molecular analysis of the hemagglutinin genes of Australian H7N7 influenza viruses: role of passerine birds in maintenance or transmission?
    Virology. 1987 Oct;160(2):411-8 PMID: 3660587
  8. Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virus.
    Cell. 1988 Apr 8;53(1):55-67 PMID: 2450679
  9. Sequence of the hemagglutinin gene from influenza virus A/Seal/Mass/1/80.
    Virology. 1983 Sep;129(2):298-308 PMID: 6688691
  10. Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41.
    Virus Res. 1986 Feb;4(2):133-43 PMID: 3010595
  11. Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector.
    EMBO J. 1986 Jun;5(6):1359-65 PMID: 3015601
  12. Correct proteolytic cleavage is required for the cell adhesive function of uvomorulin.
    J Cell Biol. 1990 Oct;111(4):1645-50 PMID: 2211831
  13. Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes.
    Nature. 1985 Feb 28-Mar 6;313(6005):756-61 PMID: 2983222
  14. Cloning and characterization of two cDNAs coding for human von Willebrand factor.
    Proc Natl Acad Sci U S A. 1985 Oct;82(19):6394-8 PMID: 2864688
  15. Inhibition of proteolytic cleavage of the hemagglutinin of influenza virus by the calcium-specific ionophore A23187.
    EMBO J. 1984 Dec 1;3(12):2911-5 PMID: 6441705
  16. Complete nucleotide sequence of an influenza virus haemagglutinin gene from cloned DNA.
    Nature. 1979 Nov 29;282(5738):471-7 PMID: 503226
  17. Is virulence of H5N2 influenza viruses in chickens associated with loss of carbohydrate from the hemagglutinin?
    Virology. 1984 Dec;139(2):303-16 PMID: 6516214
  18. Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1434-8 PMID: 2646633
  19. Proteolytic activation of the influenza virus hemagglutinin: The structure of the cleavage site and the enzymes involved in cleavage.
    Virology. 1981 Dec;115(2):361-74 PMID: 7032055
  20. The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA.
    Nucleic Acids Res. 1985 Dec 20;13(24):8765-85 PMID: 3001650
  21. Human fur gene encodes a yeast KEX2-like endoprotease that cleaves pro-beta-NGF in vivo.
    J Cell Biol. 1990 Dec;111(6 Pt 2):2851-9 PMID: 2269657
  22. Mutations at the cleavage site of the hemagglutinin after the pathogenicity of influenza virus A/chick/Penn/83 (H5N2).
    Virology. 1989 Feb;168(2):274-80 PMID: 2916326
  23. Structural variation occurring in the hemagglutinin of influenza virus A/turkey/Oregon/71 during adaptation to different cell types.
    Virology. 1990 Jun;176(2):531-8 PMID: 2345964
  24. Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptide.
    Virology. 1975 Dec;68(2):440-54 PMID: 128196
  25. Pneumotropic revertants derived from a pantropic mutant, F1-R, of Sendai virus.
    Virology. 1991 Sep;184(1):227-34 PMID: 1651590
  26. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  27. Insulin-like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.
    EMBO J. 1986 Oct;5(10):2503-12 PMID: 2877871
  28. Identification of the human cytomegalovirus glycoprotein B gene and induction of neutralizing antibodies via its expression in recombinant vaccinia virus.
    EMBO J. 1986 Nov;5(11):3057-63 PMID: 3024973
  29. An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo.
    EMBO J. 1990 Dec;9(12):4189-95 PMID: 2174359
  30. Inhibition of proteolytic activation of influenza virus hemagglutinin by specific peptidyl chloroalkyl ketones.
    Virology. 1989 Sep;172(1):25-31 PMID: 2773317
  31. Generation of seal influenza virus variants pathogenic for chickens, because of hemagglutinin cleavage site changes.
    J Virol. 1990 Jul;64(7):3297-303 PMID: 2191148
  32. Increased viral pathogenicity after insertion of a 28S ribosomal RNA sequence into the haemagglutinin gene of an influenza virus.
    Nature. 1989 Jul 13;340(6229):156-7 PMID: 2544809
  33. A host range mutant of Newcastle disease virus with an altered cleavage site for proteolytic activation of the F protein.
    Virus Res. 1990 Mar;15(3):237-42 PMID: 2188464
  34. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  35. Human influenza virus hemagglutinin with high sensitivity to proteolytic activation.
    J Virol. 1991 Jul;65(7):3530-7 PMID: 2041080
  36. Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus.
    Virology. 1987 May;158(1):242-7 PMID: 3576973
Article Info
Journal
Virology
Abbr.
Virology
ISSN
0042-6822
DOI
10.1016/0042-6822(92)90775-k
Published
1992-05-00
Pages
408-13
Language
English
Region
United States
NLM ID
0110674
PMCID
PMC7172898
Subset
IM
External Links
PubMed source DOI: 10.1016/0042-6822(92)90775-k PMC Full Text View journal details
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