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PMID: 1572985 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates.

Journal of clinical microbiology ·Vol. 30 ·No. 4 ·1992-04-00 ·Pages 967-73

Prevost G, Jaulhac B, Piemont Y

Abstract

Pulsed-field gel electrophoresis (PFGE) after SmaI restriction of DNA from 239 methicillin-resistant Staphylococcus aureus isolates (from 142 patients) produced 26 different fingerprints. The deduced chromosome sizes ranged from 2,200 to 3,100 kb (+/- 100 kb). A total of 81 isolates taken from 65 patients were then typed by PFGE and ribotyping with ClaI, EcoRI, and HindIII. Ribotypes were less discriminating than PFGE. Ribotyping did not discriminate isolates from a given PFGE fingerprint into different subsets. PFGE may be a more effective epidemiological tool than ribotyping for the typing of methicillin-resistant S. aureus strains.

MeSH Terms
Bacterial Typing Techniques DNA Fingerprinting/methods DNA, Bacterial/genetics,isolation & purification Electrophoresis, Gel, Pulsed-Field Evaluation Studies as Topic Humans Methicillin Resistance/genetics Staphylococcus aureus/classification,drug effects,genetics
Chemicals
DNA, Bacterial
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Prevost G
Institut de Bactériologie de la Faculté de Médecine, Strasbourg, France.
Jaulhac B
Piemont Y
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18 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.4.967-973.1992
Published
1992-04-00
Pages
967-73
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265195
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.4.967-973.1992 PMC Full Text View journal details
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