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PMID: 15731336 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Evolutionarily conserved human targets of adenosine to inosine RNA editing.

Nucleic acids research ·Vol. 33 ·No. 4 ·2005-00-00 ·Pages 1162-8

Levanon EY, Hallegger M, Kinar Y, Shemesh R, Djinovic-Carugo K, Rechavi G, Jantsch MF, Eisenberg E

Abstract

A-to-I RNA editing by ADARs is a post-transcriptional mechanism for expanding the proteomic repertoire. Genetic recoding by editing was so far observed for only a few mammalian RNAs that are predominantly expressed in nervous tissues. However, as these editing targets fail to explain the broad and severe phenotypes of ADAR1 knockout mice, additional targets for editing by ADARs were always expected. Using comparative genomics and expressed sequence analysis, we identified and experimentally verified four additional candidate human substrates for ADAR-mediated editing: FLNA, BLCAP, CYFIP2 and IGFBP7. Additionally, editing of three of these substrates was verified in the mouse while two of them were validated in chicken. Interestingly, none of these substrates encodes a receptor protein but two of them are strongly expressed in the CNS and seem important for proper nervous system function. The editing pattern observed suggests that some of the affected proteins might have altered physiological properties leaving the possibility that they can be related to the phenotypes of ADAR1 knockout mice.

MeSH Terms
Adenosine/metabolism Adenosine Deaminase/metabolism Amino Acid Substitution Animals Chickens/genetics Contractile Proteins/chemistry,genetics Evolution, Molecular Filamins Genomics/methods Humans Inosine/metabolism Insulin-Like Growth Factor Binding Proteins/genetics Mice Microfilament Proteins/chemistry,genetics Models, Molecular Molecular Sequence Data Neoplasm Proteins/genetics RNA Editing RNA, Messenger/chemistry,metabolism RNA-Binding Proteins
Chemicals
BLCAP protein, human Contractile Proteins Filamins Insulin-Like Growth Factor Binding Proteins Microfilament Proteins Neoplasm Proteins RNA, Messenger RNA-Binding Proteins insulin-like growth factor binding protein-related protein 1 Inosine ADARB1 protein, human Adenosine Deaminase Adenosine
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Levanon Erez Y
Compugen Ltd 72 Pinchas Rosen St, Tel-Aviv 69512, Israel. [email protected]
Hallegger Martina
Kinar Yaron
Shemesh Ronen
Djinovic-Carugo Kristina
Rechavi Gideon
Jantsch Michael F
Eisenberg Eli
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gki239
Email
[email protected]
Published
2005-00-00
Epub
2005-00-24
Pages
1162-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC549564
Subset
IM
Databases
GENBANK
AF208053
External Links
PubMed source DOI: 10.1093/nar/gki239 PMC Full Text View journal details
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