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PMID: 15772288 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sites and regulation of auxin biosynthesis in Arabidopsis roots.

The Plant cell ·Vol. 17 ·No. 4 ·2005-04-00 ·Pages 1090-104

Ljung K, Hull AK, Celenza J, Yamada M, Estelle M, Normanly J, Sandberg G

Abstract

Auxin has been shown to be important for many aspects of root development, including initiation and emergence of lateral roots, patterning of the root apical meristem, gravitropism, and root elongation. Auxin biosynthesis occurs in both aerial portions of the plant and in roots; thus, the auxin required for root development could come from either source, or both. To monitor putative internal sites of auxin synthesis in the root, a method for measuring indole-3-acetic acid (IAA) biosynthesis with tissue resolution was developed. We monitored IAA synthesis in 0.5- to 2-mm sections of Arabidopsis thaliana roots and were able to identify an important auxin source in the meristematic region of the primary root tip as well as in the tips of emerged lateral roots. Lower but significant synthesis capacity was observed in tissues upward from the tip, showing that the root contains multiple auxin sources. Root-localized IAA synthesis was diminished in a cyp79B2 cyp79B3 double knockout, suggesting an important role for Trp-dependent IAA synthesis pathways in the root. We present a model for how the primary root is supplied with auxin during early seedling development.

MeSH Terms
Arabidopsis/growth & development,metabolism Biological Assay/methods Cell Differentiation/genetics Cytochrome P-450 Enzyme System/genetics,metabolism Gene Expression Regulation, Plant/genetics Growth Inhibitors/pharmacology Indoleacetic Acids/analysis,metabolism Meristem/growth & development,metabolism Molecular Sequence Data Mutation/genetics Plant Roots/chemistry,growth & development,metabolism Seedlings/growth & development,metabolism
Chemicals
Growth Inhibitors Indoleacetic Acids indoleacetic acid Cytochrome P-450 Enzyme System cytochrome P-450 CYP79B2, Sinapis alba cytochrome P-450 CYP79B3, Sinapis alba
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ljung Karin
Umeå Plant Science Center, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-901 83, Umeå, Sweden.
Hull Anna K
Celenza John
Yamada Masashi
Estelle Mark
Normanly Jennifer
Sandberg Göran
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.104.029272
Published
2005-04-00
Epub
2005-00-16
Pages
1090-104
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1087988
Subset
IM
Grants
NIGMS NIH HHS · R01 GM043644 · United States
NIGMS NIH HHS · GM-43644 · United States
Databases
RefSeq
NM_120158, NM_127798
External Links
PubMed source DOI: 10.1105/tpc.104.029272 PMC Full Text View journal details
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