Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes.

The Journal of experimental medicine ·Vol. 134 ·No. 3 Pt 1 ·1971-09-01 ·Pages 577-87

Kappler JW, Hoffmann M, Dutton RW

Abstract

The effect of passively transfered antiserum against sheep erythrocytes (SRBC) on the antigen stimulated increase of SRBC-specific plaque-forming cells (anti-SRBC-PFC) and SRBC-specific thymus-derived lymphocytes (SRBC-specific T-cells) in the mouse spleen was examined. A dose of antiserum which severely suppressed the development of anti-SRBC-PFC did not prevent the increase in SRBC-specific T-cells, as measured by their ability to cooperate in the in vitro response to trinitrophenylated (TNP) SRBC. It was shown that the insensitivity of these T-cells to antiserum could not be explained by their low antigen requirement as compared to that of PFC. In the in vivo response of mice to TNP-SRBC, antibody specific for TNP suppressed the appearance of both anti-TNP- and anti-SRBC-PFC. The presence of free SRBC specifically prevented the suppression of the anti-SRBC-PFC. These observations are consistent with opsonization by phagocytic cells as the primary means of the observed suppression of PFC development by antibody.

MeSH Terms

Animals Antibody Formation Antibody-Producing Cells/immunology Bone Marrow/immunology Erythrocytes/immunology Female Immune Sera/immunology Immune Tolerance Male Mice Mice, Inbred C57BL Mice, Inbred DBA Sheep T-Lymphocytes/immunology Thymus Gland/immunology

Chemicals

Immune Sera

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kappler J W

Hoffmann M

Dutton R W

References (27)

27 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.134.3.577

Published

1971-09-01

Pages

577-87

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139081

Subset

External Links

PubMed source DOI: 10.1084/jem.134.3.577 PMC Full Text View journal details

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Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes.

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