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PMID: 1579459 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

Nucleic acids research ·Vol. 20 ·No. 7 ·1992-04-11 ·Pages 1679-84

Maskos U, Southern EM

Abstract

A novel linker for the synthesis of oligonucleotides on a glass support is described. Oligonucleotides synthesised on the support remain tethered to the support after ammonia treatment and are shown to take part in sequence specific hybridisation reactions. These hybridizations were carried out with oligonucleotides synthesised on 'ballotini' solid sphere glass beads and microscope slides. The linker has a hexaethylene glycol spacer, bound to the glass via a glycidoxypropyl silane, terminating in a primary hydroxyl group that serves as starting point for automated or manual oligonucleotide synthesis.

MeSH Terms

Base Sequence Chromatography Glass Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotides/chemical synthesis,metabolism Polyethylene Glycols Silanes

Chemicals

Oligonucleotides Silanes Polyethylene Glycols

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Maskos U

Department of Biochemistry, University of Oxford, UK.

Southern E M

References (14)

14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/20.7.1679

Published

1992-04-11

Pages

1679-84

Language

English

Region

England

NLM ID

0411011

PMCID

PMC312256

Subset

External Links

PubMed source DOI: 10.1093/nar/20.7.1679 PMC Full Text View journal details

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Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.

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