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PMID: 3601674 Published · ppublish English Journal Article

Covalent attachment of oligonucleotides to solid supports.

Nucleic acids research ·Vol. 15 ·No. 13 ·1987-07-10 ·Pages 5353-72

Ghosh SS, Musso GF

Abstract

Coupling efficiencies for the covalent attachment of oligonucleotides (17-29 bases in length) to solid supports derivatized with alkyl-amino and -carboxylic functionalities have been determined. Attachment efficiencies of 60-80% were obtained for coated long-chain alkylamino controlled pore glass (CPG) supports. Similar efficiencies of immobilization were observed for carboxyl-bearing supports, which additionally exhibited lower levels of non-covalent binding. The extent of terminally linked oligonucleotide was determined to be 50-55% of the overall attachment in the carbodiimide-mediated coupling reaction of a 5'-aminohexyl phosphoramidate derivative of a 29-mer to Sephacryl carboxyl support. While lower overall efficiencies of attachment were obtained in the reaction with Sephacryl N-hydroxysuccinimide-activated carboxyl support, greater than 80% of this coupling results in end-attached oligonucleotides.

MeSH Terms
Chemical Phenomena Chemistry Glass Indicators and Reagents Oligonucleotides Polynucleotide 5'-Hydroxyl-Kinase RNA, Transfer Resins, Plant T-Phages/enzymology
Chemicals
Indicators and Reagents Oligonucleotides Resins, Plant RNA, Transfer Polynucleotide 5'-Hydroxyl-Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ghosh S S
Musso G F
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.13.5353
Published
1987-07-10
Pages
5353-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC305966
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.13.5353 PMC Full Text View journal details
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