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PMID: 15805194 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A checkpoint control linking meiotic S phase and recombination initiation in fission yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 16 ·2005-04-19 ·Pages 5797-801

Tonami Y, Murakami H, Shirahige K, Nakanishi M

Abstract

During meiosis, high levels of recombination initiated by DNA double-strand breaks (DSBs) occur only after DNA replication. However, how DSB formation is coupled to DNA replication is unknown. We examined several DNA replication proteins for a role in this coupling in Schizosaccharomyces pombe, and we show that ribonucleotide reductase, the rate-limiting enzyme of deoxyribonucleotide synthesis and the target of the DNA synthesis inhibitor hydroxyurea (HU) is indirectly required for DSB formation linked to DNA replication. However, in cells in which the function of the DNA-replication-checkpoint proteins Rad1p, Rad3p, Rad9p, Rad17p, Rad26p, Hus1p, or Cds1p was compromised, DSB formation occurred at similar frequencies in the absence or presence of HU. The DSBs in the HU-treated mutant cells occurred at normal sites and were associated with recombination. In addition, Cdc2p is apparently not involved in this process. We propose that the sequence of meiotic S phase and initiation of recombination is coordinated by DNA-replication-checkpoint proteins.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism DNA Replication Genes, cdc Hydroxyurea/metabolism Meiosis/physiology Nucleic Acid Synthesis Inhibitors/metabolism Recombination, Genetic Schizosaccharomyces/cytology,physiology Schizosaccharomyces pombe Proteins/genetics,metabolism
Chemicals
Cell Cycle Proteins Nucleic Acid Synthesis Inhibitors Schizosaccharomyces pombe Proteins Hydroxyurea
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tonami Yuko
Department of Biochemistry and Cell Biology, Graduate School of Medicine, Nagoya City University, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan.
Murakami Hiroshi
Shirahige Katsuhiko
Nakanishi Makoto
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0407236102
Published
2005-04-19
Epub
2005-00-01
Pages
5797-801
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC556284
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0407236102 PMC Full Text View journal details
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