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PMID: 1583717 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Effects of deletions in the cytoplasmic domain on biological functions of human immunodeficiency virus type 1 envelope glycoproteins.

Journal of virology ·Vol. 66 ·No. 6 ·1992-06-00 ·Pages 3306-15

Gabuzda DH, Lever A, Terwilliger E, Sodroski J

Abstract

The role of the cytoplasmic domain of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins in virus replication was investigated. Deletion of residues 840 to 856 at the carboxyl terminus of gp41 reduced the efficiency of virus entry during an early step in the virus life cycle between CD4 binding and formation of the DNA provirus without affecting envelope glycoprotein synthesis, processing, or syncytium-forming ability. Deletion of residues amino terminal to residue 846 was associated with decreased stability of envelope glycoproteins made in COS-1 cells, but this phenotype was cell type dependent. The cytoplasmic domain of gp41 was not required for the incorporation of the HIV-1 envelope glycoproteins into virions. These results suggest that the carboxyl terminus of the gp41 cytoplasmic domain plays a role in HIV-1 entry other than receptor binding or membrane fusion. The cytoplasmic domain of gp41 also affects the stability of the envelope glycoprotein in some cell types.

MeSH Terms
Acquired Immunodeficiency Syndrome/genetics Base Sequence CD4 Antigens/metabolism Cells, Cultured DNA Mutational Analysis Giant Cells/microbiology HIV Envelope Protein gp41/genetics HIV-1/genetics,growth & development Molecular Sequence Data Mutation/genetics Protein Conformation Protein Processing, Post-Translational Proviruses/growth & development T-Lymphocytes/microbiology Viral Envelope Proteins/biosynthesis Virion/growth & development Virus Replication/genetics
Chemicals
CD4 Antigens HIV Envelope Protein gp41 Viral Envelope Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gabuzda D H
Division of Human Retrovirology, Dana-Farber Cancer Institute, Boston, Massachusetts 02115.
Lever A
Terwilliger E
Sodroski J
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.6.3306-3315.1992
Published
1992-06-00
Pages
3306-15
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241108
Subset
IM
Grants
NIAID NIH HHS · AI01017 · United States
NIAID NIH HHS · AI24755 · United States
NIAID NIH HHS · AI28193 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.6.3306-3315.1992 PMC Full Text View journal details
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