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PMID: 1588275 Published · ppublish English Journal Article

The MRC OX-62 antigen: a useful marker in the purification of rat veiled cells with the biochemical properties of an integrin.

The Journal of experimental medicine ·Vol. 175 ·No. 6 ·1992-06-01 ·Pages 1457-65

Brenan M, Puklavec M

Abstract

A mouse immunoglobulin G1 monoclonal antibody (mAb), MRC OX-62 (OX-62), was raised against density gradient-enriched rat veiled (dendritic) cells obtained from lymph. In suspensions of lymphoid cells, the OX-62 mAb only labeled cells with the characteristics of veiled cells. The OX-62 mAb was used with a magnetic cell sorter to enrich or deplete veiled cells, and the enriched veiled cells were potent stimulators in the primary allogeneic mixed leukocyte reaction. Immunohistochemical staining of tissue sections showed that the OX-62 mAb did not label all classical dendritic cells and was not restricted to this cell type. In lymphoid tissues, the labeling correlated with dendritic cells, but in skin, major histocompatibility complex class II+ cells were OX-62-, while another CD3+ cell with dendritic morphology was strongly OX-62+. It seems that the OX-62 mAb may be restricted to dendritic cells and probably to gamma/delta T cells. The OX-62 mAb will be of use in delineating minor subsets of cells with dendritic morphology in various tissues. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of veiled cell-enriched populations immunoprecipitated with the OX-62 mAb gave bands with the biochemical characteristics of an integrin. The OX-62 mAb recognized the alpha-like subunit.

MeSH Terms
Animals Antibodies, Monoclonal Antigens, CD Antigens, Surface/analysis Biomarkers Cell Separation/methods Dendritic Cells/cytology Flow Cytometry/methods Hybridization, Genetic Hybridomas/immunology Immunohistochemistry Integrins/analysis Leukocytes/cytology Lymphocytes/cytology Magnetics Membrane Glycoproteins/analysis Mice Mice, Inbred BALB C Mice, Inbred DBA Organ Specificity Rats Rats, Inbred Strains
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Surface Biomarkers Integrins Membrane Glycoproteins antigens, CD200
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brenan M
Medical Research Council Cellular Immunology Unit, Sir William Dunn School of Pathology, University of Oxford, England.
Puklavec M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.175.6.1457
Published
1992-06-01
Pages
1457-65
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2119258
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.175.6.1457 PMC Full Text View journal details
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