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PMID: 2663881 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Integrin heterodimer and receptor complexity in avian and mammalian cells.

The Journal of cell biology ·Vol. 109 ·No. 1 ·1989-07-00 ·Pages 409-20

Hynes RO, Marcantonio EE, Stepp MA, Urry LA, Yee GH

Abstract

We report data showing that the integrin receptor complex in chickens contains several discrete heterodimers all sharing the beta 1-integrin subunit combined separately with different alpha-subunits. Using antisera to synthetic peptides based on cDNA sequences of chicken and human alpha-integrin subunits to analyze the integrin complement of avian and mammalian cells, we show that band 2 of the chicken integrin complex contains alpha-subunits related to both alpha 3- and alpha 5-subunits of human integrins. alpha 3 beta 1 and alpha 5 beta 1 have both previously been shown in human cells to be fibronectin receptors and alpha 3 beta 1 can also act as a receptor for laminin and collagen. We also provide evidence for the presence, in band 1 of the chicken integrin complex, of a third integrin alpha-subunit which is also alpha 5 related. This integrin subunit exists in a separate heterodimer complex with beta 1 and binds to fibronectin-affinity columns. These results provide explanations for published data showing that the avian integrin complex contains receptor activity for a variety of extracellular matrix proteins. We conclude that the chicken integrin complex comprises a set of beta 1-integrin heterodimers equivalent to the human VLA antigens and includes at least two fibronectin receptors. Finally, we show that chicken embryo fibroblasts also contain a beta 3-class integrin related to the RGD receptors defined in various human cells.

MeSH Terms
Animals Blotting, Western Cell Adhesion Chickens Chromatography, Affinity Cloning, Molecular DNA/genetics Fibronectins/metabolism Humans Immunologic Techniques Integrins Ligands Macromolecular Substances Membrane Glycoproteins/physiology Molecular Sequence Data Molecular Weight Multigene Family
Chemicals
Fibronectins Integrins Ligands Macromolecular Substances Membrane Glycoproteins DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hynes R O
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Marcantonio E E
Stepp M A
Urry L A
Yee G H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.109.1.409
Published
1989-07-00
Pages
409-20
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115488
Subset
IM
Grants
NCI NIH HHS · F32CA08140 · United States
NCI NIH HHS · R01 CA 17007 · United States
External Links
PubMed source DOI: 10.1083/jcb.109.1.409 PMC Full Text View journal details
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