Human interferon: mass production in a newly established cell line, MG-63.

Antimicrobial agents and chemotherapy ·Vol. 12 ·No. 1 ·1977-07-00 ·Pages 11-5

Billiau A, Edy VG, Heremans H, Van Damme J, Desmyter J, Georgiades JA, De Somer P

Abstract

MG-63 cells, a line derived from an osteosarcoma, produced high yields of interferon after superinduction with polyinosinic acid.polycytidylic acid, cycloheximide, and actinomycin D. Advantages of MG-63 cells over diploid fibroblasts as a substrate are: no requirement for aging between confluency and induction, no requirement for priming, and 3.7-fold higher yields per square centimeter of culture surface. Physicochemically and biologically, MG-63 cell interferon resembles fibroblast rather than leukocyte interferon.

MeSH Terms

Cell Line Cells, Cultured Diploidy Fibroblasts/metabolism Humans In Vitro Techniques Interferons/biosynthesis Neoplasms/metabolism Neutralization Tests Skin/cytology

Chemicals

Interferons

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Billiau A

Edy V G

Heremans H

Van Damme J

Desmyter J

Georgiades J A

De Somer P

References (14)

14 references, click to expand

Purification of interferon by adsorption chromatography on controlled pore glass.
J Gen Virol. 1976 Dec;33(3):517-21 PMID: 1036458
Pronounced antiviral activity of human interferon on bovine and porcine cells.
Nature. 1974 Oct 11;251(5475):543-5 PMID: 4371444
Mass production of human interferon in diploid cells stimulated by poly-I:C.
J Gen Virol. 1973 Apr;19(1):1-8 PMID: 4360630
Administration of human fibroblast interferon in chronic hepatitis-B infection.
Lancet. 1976 Sep 25;2(7987):645-7 PMID: 60513
Production of human lymphoblastoid interferon.
J Clin Microbiol. 1975 Jan;1(1):116-7 PMID: 170301
Two antigenically distinct species of human interferon.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2185-7 PMID: 49055
Regulation of cellular interferon production: enhancement by antimetabolites.
Proc Natl Acad Sci U S A. 1970 Sep;67(1):464-71 PMID: 5272327
Human leukocyte interferon: production, purification, stability, and animal experiments.
In Vitro Monogr. 1974;(3):35-8 PMID: 4620359
The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5.
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2251-5 PMID: 4526303
Mass production of human interferon in diploid cells.
In Vitro Monogr. 1974;(3):39-46 PMID: 4471020
Production of high-titered interferon in cultures of human diploid cells.
Antimicrob Agents Chemother. 1972 Dec;2(6):476-84 PMID: 4670440
Raising antibodies to human leukocyte interferon.
Acta Pathol Microbiol Scand B. 1975 Oct;83(5):443-50 PMID: 1180059
In vitro cultivation of human tumor tissues.
Oncology. 1975;31(5-6):257-72 PMID: 1208022
Molecular modification of interferon: attainment of human interferon in a conformation active on cat cells but inactive on human cells.
Virology. 1976 Apr;70(2):451-8 PMID: 178096

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.12.1.11

Published

1977-07-00

Pages

11-5

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC352146

Subset

External Links

PubMed source DOI: 10.1128/AAC.12.1.11 PMC Full Text View journal details

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