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PMID: 15883201 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tight junctions in Schwann cells of peripheral myelinated axons: a lesson from claudin-19-deficient mice.

The Journal of cell biology ·Vol. 169 ·No. 3 ·2005-05-09 ·Pages 527-38

Miyamoto T, Morita K, Takemoto D, Takeuchi K, Kitano Y, Miyakawa T, Nakayama K, Okamura Y, Sasaki H, Miyachi Y, Furuse M, Tsukita S

Abstract

Tight junction (TJ)-like structures have been reported in Schwann cells, but their molecular composition and physiological function remain elusive. We found that claudin-19, a novel member of the claudin family (TJ adhesion molecules in epithelia), constituted these structures. Claudin-19-deficient mice were generated, and they exhibited behavioral abnormalities that could be attributed to peripheral nervous system deficits. Electrophysiological analyses showed that the claudin-19 deficiency affected the nerve conduction of peripheral myelinated fibers. Interestingly, the overall morphology of Schwann cells lacking claudin-19 expression appeared to be normal not only in the internodal region but also at the node of Ranvier, except that TJs completely disappeared, at least from the outer/inner mesaxons. These findings have indicated that, similar to epithelial cells, Schwann cells also bear claudin-based TJs, and they have also suggested that these TJs are not involved in the polarized morphogenesis but are involved in the electrophysiological "sealing" function of Schwann cells.

MeSH Terms
Animals Axons/metabolism,pathology,ultrastructure Cell Membrane/metabolism,pathology,ultrastructure Claudins Female Male Membrane Proteins/genetics,metabolism Mice Mice, Inbred C57BL Mice, Knockout Myelin Sheath/metabolism,pathology,ultrastructure Nerve Fibers, Myelinated/metabolism,pathology,ultrastructure Neural Conduction/genetics Peripheral Nerves/abnormalities,metabolism,ultrastructure Ranvier's Nodes/metabolism,pathology,ultrastructure Schwann Cells/metabolism,pathology,ultrastructure Tight Junctions/metabolism,pathology,ultrastructure
Chemicals
Claudins Cldn19 protein, mouse Membrane Proteins
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Miyamoto Tatsuo
Department of Cell Biology, Graduate School of Medicine, Kyoto University, Japan.
Morita Kazumasa
Takemoto Daisuke
Takeuchi Kosei
Kitano Yuka
Miyakawa Tsuyoshi
Nakayama Kiyomi
Okamura Yasushi
Sasaki Hiroyuki
Miyachi Yoshiki
Furuse Mikio
Tsukita Shoichiro
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200501154
Published
2005-05-09
Pages
527-38
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171943
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200501154 PMC Full Text View journal details
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