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PMID: 1592809 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Membrane intermediates in the peptidoglycan metabolism of Escherichia coli: possible roles of PBP 1b and PBP 3.

Journal of bacteriology ·Vol. 174 ·No. 11 ·1992-06-00 ·Pages 3549-57

van Heijenoort Y, Gómez M, Derrien M, Ayala J, van Heijenoort J

Abstract

The two membrane precursors (pentapeptide lipids I and II) of peptidoglycan are present in Escherichia coli at cell copy numbers no higher than 700 and 2,000 respectively. Conditions were determined for an optimal accumulation of pentapeptide lipid II from UDP-MurNAc-pentapeptide in a cell-free system and for its isolation and purification. When UDP-MurNAc-tripeptide was used in the accumulation reaction, tripeptide lipid II was formed, and it was isolated and purified. Both lipids II were compared as substrates in the in vitro polymerization by transglycosylation assayed with PBP 1b or PBP 3. With PBP 1b, tripeptide lipid II was used as efficiently as pentapeptide lipid II. It should be stressed that the in vitro PBP 1b activity accounts for at best to 2 to 3% of the in vivo synthesis. With PBP 3, no polymerization was observed with either substrate. Furthermore, tripeptide lipid II was detected in D-cycloserine-treated cells, and its possible in vivo use in peptidoglycan formation is discussed. In particular, it is speculated that the transglycosylase activity of PBP 1b could be coupled with the transpeptidase activity of PBP 3, using mainly tripeptide lipid II as precursor.

MeSH Terms
Bacterial Proteins Carrier Proteins Cell Membrane/metabolism Dipeptides/metabolism Escherichia coli/metabolism Glycosylation Hexosyltransferases/isolation & purification,metabolism Multienzyme Complexes/isolation & purification,metabolism Muramoylpentapeptide Carboxypeptidase Penicillin-Binding Proteins Peptidoglycan/biosynthesis Peptidyl Transferases/isolation & purification,metabolism Polyisoprenyl Phosphate Monosaccharides/metabolism Polyisoprenyl Phosphate Oligosaccharides/isolation & purification,metabolism Uridine Diphosphate N-Acetylmuramic Acid/analogs & derivatives,metabolism
Chemicals
Bacterial Proteins Carrier Proteins Dipeptides Multienzyme Complexes Penicillin-Binding Proteins Peptidoglycan Polyisoprenyl Phosphate Monosaccharides Polyisoprenyl Phosphate Oligosaccharides Uridine Diphosphate N-Acetylmuramic Acid N-acetylglucosaminyl-N-acetylmuramyl(tripeptide)-pyrophosphate-undecaprenol UDP-N-acetylmuramic acid pentapeptide alanylalanine undecaprenyl diphosphate-(N-acetylglucosaminyl)(1-4)-N-acetylmuramoyl pentapeptide undecaprenyl biphosphate-N-acetylmuramoyl-pentapeptide Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
van Heijenoort Y
Centre National de la Recherche Scientifique, Université Paris-Sud, Orsay, France.
Gómez M
Derrien M
Ayala J
van Heijenoort J
References (43)
43 references, click to expand
  1. On the role of the high molecular weight penicillin-binding proteins in the cell cycle of Escherichia coli.
    Res Microbiol. 1991 Feb-Apr;142(2-3):333-44 PMID: 1925032
  2. Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence.
    Biochem Biophys Res Commun. 1981 Aug 14;101(3):905-11 PMID: 7030331
  3. The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide.
    J Bacteriol. 1990 Dec;172(12):6697-703 PMID: 2254246
  4. Differential effect of mutational impairment of penicillin-binding proteins 1A and 1B on Escherichia coli strains harboring thermosensitive mutations in the cell division genes ftsA, ftsQ, ftsZ, and pbpB.
    J Bacteriol. 1990 Oct;172(10):5863-70 PMID: 2211517
  5. Cell division and peptidoglycan assembly in Escherichia coli.
    Mol Microbiol. 1991 Apr;5(4):791-5 PMID: 1649945
  6. The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis.
    J Bacteriol. 1991 Aug;173(15):4625-36 PMID: 1649817
  7. The Escherichia coli mraY gene encoding UDP-N-acetylmuramoyl-pentapeptide: undecaprenyl-phosphate phospho-N-acetylmuramoyl-pentapeptide transferase.
    J Bacteriol. 1991 Feb;173(3):1021-6 PMID: 1846850
  8. Autolysis-resistant peptidoglycan of anomalous composition in amino-acid-starved Escherichia coli.
    J Bacteriol. 1988 Mar;170(3):1373-6 PMID: 2893787
  9. Incorporation of LL-diaminopimelic acid into peptidoglycan of Escherichia coli mutants lacking diaminopimelate epimerase encoded by dapF.
    J Bacteriol. 1988 May;170(5):2031-9 PMID: 3283102
  10. Variability in the posttranslational processing of penicillin-binding protein 1b among different strains of Escherichia coli.
    Biochem Cell Biol. 1987 Jan;65(1):62-7 PMID: 3552002
  11. Activity of penicillin-binding protein 3 from Escherichia coli.
    J Bacteriol. 1986 Oct;168(1):199-206 PMID: 3531167
  12. Effect of growth conditions on peptidoglycan content and cytoplasmic steps of its biosynthesis in Escherichia coli.
    J Bacteriol. 1985 Jul;163(1):208-12 PMID: 3891726
  13. Penicillin-binding proteins of gram-negative bacteria.
    Rev Infect Dis. 1988 Jul-Aug;10(4):699-711 PMID: 3055170
  14. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  15. Separation and quantification of muropeptides with high-performance liquid chromatography.
    Anal Biochem. 1988 Aug 1;172(2):451-64 PMID: 3056100
  16. The composition of the murein of Escherichia coli.
    J Biol Chem. 1988 Jul 25;263(21):10088-95 PMID: 3292521
  17. Determination of murein precursors during the cell cycle of Escherichia coli.
    J Gen Microbiol. 1989 Jun;135(6):1499-506 PMID: 2693601
  18. Variations in UDP-N-acetylglucosamine and UDP-N-acetylmuramyl-pentapeptide pools in Escherichia coli after inhibition of protein synthesis.
    J Bacteriol. 1989 Jun;171(6):3282-7 PMID: 2656647
  19. Functional biosynthesis of cell wall peptidoglycan by polymorphic bifunctional polypeptides. Penicillin-binding protein 1Bs of Escherichia coli with activities of transglycosylase and transpeptidase.
    J Biol Chem. 1984 Nov 25;259(22):13937-46 PMID: 6389538
  20. Effects of moenomycin on Escherichia coli.
    J Gen Microbiol. 1987 Mar;133(3):667-74 PMID: 3309167
  21. On the process of cellular division in Escherichia coli: isolation and characterization of penicillin-binding proteins 1a, 1b, and 3.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4499-503 PMID: 7001458
  22. In vitro peptidoglycan polymerization catalysed by penicillin binding protein 1b of Escherichia coli K-12.
    FEBS Lett. 1980 Feb 11;110(2):245-9 PMID: 6989636
  23. Reverse-phase high-pressure liquid chromatography of uridine diphosphate N-acetylmuramyl peptide precursors of bacterial cell wall peptidoglycan.
    Anal Biochem. 1981 Jun;114(1):59-63 PMID: 7283154
  24. Phospho-N-acetylmuramoyl-pentapeptide-transferase of Escherichia coli K12. Properties of the membrane-bound and the extracted and partially purified enzyme.
    Biochim Biophys Acta. 1978 Dec 8;527(2):414-24 PMID: 215212
  25. Polymerization by transglycosylation in the biosynthesis of the peptidoglycan of Escherichia coli K 12 and its inhibition by antibiotics.
    FEBS Lett. 1978 May 1;89(1):141-4 PMID: 350621
  26. Involvement of the relA gene product and feedback inhibition in the regulation of DUP-N-acetylmuramyl-peptide synthesis in Escherichia coli.
    J Bacteriol. 1978 Sep;135(3):766-74 PMID: 357424
  27. Site of inhibition of peptidoglycan biosynthesis during the stringent response in Escherichia coli.
    J Bacteriol. 1978 Jul;135(1):71-7 PMID: 353038
  28. Properties of the penicillin-binding proteins of Escherichia coli K12,.
    Eur J Biochem. 1977 Jan;72(2):341-52 PMID: 319999
  29. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome.
    Cell. 1976 Sep;9(1):91-9 PMID: 788919
  30. Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus.
    J Biol Chem. 1972 Aug 25;247(16):5123-31 PMID: 5057461
  31. Murein synthesis and identification of cell wall precursors of temperature-sensitive lysis mutants of Escherichia coli.
    J Bacteriol. 1972 Jan;109(1):326-35 PMID: 4550671
  32. Isolation of polyisoprenyl alcohols from Streptococcus faecalis.
    J Bacteriol. 1972 Dec;112(3):1302-5 PMID: 4344921
  33. Release of D-alanyl-D-alanine from the precursor of the cell wall peptidoglycan by a peptidase of Escherichia coli K 12.
    Biochimie. 1973;55(6):685-91 PMID: 4589241
  34. Isolation of the lipid intermediate in peptidoglycan biosynthesis from Escherichia coli.
    J Bacteriol. 1972 Dec;112(3):1306-9 PMID: 4565540
  35. Isolation, purification, and properties of the lipid-linked intermediates of O-antigen biosynthesis.
    Arch Biochem Biophys. 1966 Sep 26;116(1):425-35 PMID: 4960204
  36. Enzymatic synthesis of cell wall mucopeptide in a particulate preparation of Escherichia coli.
    Biochem Biophys Res Commun. 1966 May 25;23(4):466-72 PMID: 5335730
  37. Activity of three murein hydrolases during the cell division cycle of Escherichia coli K-12 as measured in toluene-treated cells.
    J Bacteriol. 1976 Jun;126(3):1250-60 PMID: 780345
  38. Purification and characterization of the D-alanyl-D-alanine-adding enzyme from Escherichia coli.
    Biochemistry. 1990 Mar 6;29(9):2379-86 PMID: 2186811
  39. Machinery for cell growth and division: penicillin-binding proteins and other proteins.
    Res Microbiol. 1990 Jan;141(1):89-103 PMID: 2114032
  40. Nucleotide sequence of the regulatory region of the gene pbpB of Escherichia coli.
    Nucleic Acids Res. 1990 May 11;18(9):2813 PMID: 2187182
  41. Cytoplasmic steps of peptidoglycan synthesis in Escherichia coli.
    J Bacteriol. 1982 Sep;151(3):1109-17 PMID: 6125497
  42. Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A.
    Biochem Biophys Res Commun. 1980 Nov 17;97(1):287-93 PMID: 7006606
  43. Synthetic ColE1 Plasmids carrying genes for penicillin-binding proteins in Escherichia coli.
    Plasmid. 1981 Jul;6(1):86-98 PMID: 7025060
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.11.3549-3557.1992
Published
1992-06-00
Pages
3549-57
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206040
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/jb.174.11.3549-3557.1992 PMC Full Text View journal details
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