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PMID: 15937225 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Visualization of diffuse centromeres with centromere-specific histone H3 in the holocentric plant Luzula nivea.

The Plant cell ·Vol. 17 ·No. 7 ·2005-07-00 ·Pages 1886-93

Nagaki K, Kashihara K, Murata M

Abstract

Although holocentric species are scattered throughout the plant and animal kingdoms, only holocentric chromosomes of the nematode worm Caenorhabditis elegans have been analyzed with centromeric protein markers. In an effort to determine the holocentric structure in plants, we investigated the snowy woodrush Luzula nivea. From the young roots, a cDNA encoding a putative centromere-specific histone H3 (LnCENH3) was successfully isolated based on sequence similarity among plant CENH3s. The deduced amino acid sequence was then used to raise an anti-LnCENH3 antibody. Immunostaining clearly revealed the diffuse centromere-like structure that appears in the linear shape at prophase to telophase. Furthermore, it was shown that the amount of LnCENH3 decreased significantly at interphase. The polar side positioning on each chromatid at metaphase to anaphase also confirmed that LnCENH3 represents one of the centromere-specific proteins in L. nivea. These data from L. nivea are compared with those from C. elegans, and common features of holocentric chromosomes are discussed.

MeSH Terms
Amino Acid Sequence/genetics Antibody Specificity Base Sequence/genetics Cell Cycle/genetics Cell Nucleus/genetics,metabolism Centromere/genetics Chromatids/genetics DNA, Complementary/analysis,genetics Evolution, Molecular Histones/chemistry,genetics,isolation & purification Immunohistochemistry Interphase/genetics Magnoliopsida/genetics,metabolism Metaphase/genetics Mitosis/genetics Molecular Sequence Data Prophase/genetics Telophase/genetics
Chemicals
DNA, Complementary Histones
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nagaki Kiyotaka
Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki, 710-0046, Japan. [email protected]
Kashihara Kazunari
Murata Minoru
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.105.032961
Email
[email protected]
Published
2005-07-00
Epub
2005-00-03
Pages
1886-93
Language
English
Region
England
NLM ID
9208688
PMCID
PMC1167539
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.105.032961 PMC Full Text View journal details
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