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PMID: 12663558 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Chromatin immunoprecipitation reveals that the 180-bp satellite repeat is the key functional DNA element of Arabidopsis thaliana centromeres.

Genetics ·Vol. 163 ·No. 3 ·2003-03-00 ·Pages 1221-5

Nagaki K, Talbert PB, Zhong CX, Dawe RK, Henikoff S, Jiang J

Abstract

The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that this repeat is the key component of the centromere/kinetochore complex in Arabidopsis.

MeSH Terms
Arabidopsis/genetics Arabidopsis Proteins/genetics Centromere/genetics Chromatin/isolation & purification Cloning, Molecular DNA, Plant/genetics Microsatellite Repeats/genetics
Chemicals
Arabidopsis Proteins Chromatin DNA, Plant
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nagaki Kiyotaka
Department of Horticulture, University of Wisconsin, Madison, Wisconsin 53706, USA.
Talbert Paul B
Zhong Cathy Xiaoyan
Dawe R Kelly
Henikoff Steven
Jiang Jiming
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/163.3.1221
Published
2003-03-00
Pages
1221-5
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462492
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/163.3.1221 PMC Full Text View journal details
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