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PMID: 159455 Published · ppublish English Journal Article

Cloning of the structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 9 ·1979-09-00 ·Pages 4360-4

Henning U, Royer HD, Teather RM, Hindennach I, Hollenberg CP

Abstract

The gene (ompA) for the major outer membrane protein II* from Escherichia coli K-12 has been cloned on a 5-megadalton EcoRI fragment by using phage lambda as vector. The gene is expressed during the lytic cycle of the recombinant phage and the insoluble membrane-bound protein was detected in phage plaques with a simple radioimmunoassay. Transfer of the EcoRI fragment into plasmid pSC101 and expression in a host lacking protein II* led to overproduction of protein II* and decreased production of two other major outer membrane proteins. Expression of the plasmid pSC101-ompA+ in minicells derived from an ompA minicell-producing strain led to synthesis, at high rates, of this protein and massive accumulation of a second cell envelope protein most likely representing the biosynthetic precursor of protein II*.

MeSH Terms
Bacterial Proteins/genetics Bacteriophage lambda/genetics DNA, Bacterial/genetics DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Genes Membrane Proteins/genetics Molecular Weight Plasmids
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Recombinant DNA, Viral Membrane Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Henning U
Royer H D
Teather R M
Hindennach I
Hollenberg C P
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50 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.9.4360
Published
1979-09-00
Pages
4360-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411574
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.9.4360 PMC Full Text View journal details
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