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PMID: 1594631 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 11 ·1992-06-01 ·Pages 5192-6

Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT

Abstract

Expression of the Rous sarcoma virus-encoded oncoprotein, pp60v-src, subverts the normal regulation of cell growth, which results in oncogenic transformation. This process requires the intrinsic protein-tyrosine kinase activity of pp60v-src and is associated with an increase in tyrosine phosphorylation of a number of cellular proteins, candidate substrates for pp60v-src. We report here the isolation of a cDNA encoding a protein, pp125, that is a major phosphotyrosine-containing protein in untransformed chicken embryo cells and exhibits an increase in phosphotyrosine in pp60v-src-transformed chicken embryo cells. This cDNA encodes a cytoplasmic protein-tyrosine kinase which, based upon its predicted amino acid sequence and structure, is the prototype for an additional family of protein-tyrosine kinases. Immunofluorescence localization experiments show that pp125 is localized to focal adhesions; hence, we suggest the name focal adhesion kinase.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Adhesion Cell Adhesion Molecules/genetics,metabolism Cell Transformation, Viral Chick Embryo Cloning, Molecular Cytoskeletal Proteins/metabolism DNA/genetics Fluorescent Antibody Technique Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Microfilament Proteins Molecular Sequence Data Molecular Weight Phosphoproteins/genetics Protein-Tyrosine Kinases/genetics,metabolism Restriction Mapping Sequence Alignment Tensins
Chemicals
Cell Adhesion Molecules Cytoskeletal Proteins Microfilament Proteins Phosphoproteins Tensins DNA Protein-Tyrosine Kinases Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schaller M D
Department of Microbiology, School of Medicine, University of Virginia, Charlottesville 22908.
Borgman C A
Cobb B S
Vines R R
Reynolds A B
Parsons J T
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.11.5192
Published
1992-06-01
Pages
5192-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49256
Subset
IM
Grants
NCI NIH HHS · CA29243 · United States
NCI NIH HHS · CA40042 · United States
Databases
GENBANK
M86656
External Links
PubMed source DOI: 10.1073/pnas.89.11.5192 PMC Full Text View journal details
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