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PMID: 15981335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The halo-opsin gene. I. Identification and isolation.

The EMBO journal ·Vol. 6 ·No. 1 ·1987-01-00 ·Pages 259-64

Hegemann P, Blanck A, Vogelsang-Wenke H, Lottspeich F, Oesterhelt D

Abstract

Halorhodopsin (HR), the light-driven chloride pump in halobacteria, was purified in the denatured as well as in the native state and chemically cleaved into peptide fragments. Isolation of peptide and liquid phase sequencing yielded approximately 20% of the halo-opsin (HO) structure in non-overlapping peptides. Chemically synthesized oligodeoxynucleotides corresponding to a peptide sequence obtained from both HR preparations were used to screen a cosmid gene bank of Halobacterium halobium strain L-33. A positive clone contained cosmid pAB H47 which by subcloning and nucleotide sequencing was shown to encode at least part of the HO gene.

MeSH Terms
Blotting, Southern Chromatography, High Pressure Liquid Cosmids/genetics Genes, Bacterial/genetics Genomic Library Halobacterium salinarum/genetics Halorhodopsins/genetics,isolation & purification
Chemicals
Halorhodopsins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hegemann P
Max-Planck Institut für Biochemie, D-8033, Martinsried, FRG.
Blanck A
Vogelsang-Wenke H
Lottspeich F
Oesterhelt D
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1987-01-00
Pages
259-64
Language
English
Region
England
NLM ID
8208664
PMCID
PMC553386
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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