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PMID: 15981336 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin.

The EMBO journal ·Vol. 6 ·No. 1 ·1987-01-00 ·Pages 265-73

Blanck A, Oesterhelt D

Abstract

The gene for the protein moiety of the light-driven chloride pump halorhodopsin (HR), hop gene, was sequenced and the primary structure of the protein derived thereof. The gene has a GC content of 67% and codes for 274 amino acids. A promoter structure, resembling that of the halobacterial 16S rRNA genes, is present and both a terminating stem and a loop sequence is found downstream of the TGA stop codon. A ribosomal binding site is located within the translated region. The HR protein moiety is processed at the amino terminus, as well as the carboxy terminus, yielding a dominant species of calculated Mr 26 961.

MeSH Terms

Amino Acid Sequence Bacteriorhodopsins/genetics Base Sequence Genes, Bacterial/genetics Halorhodopsins/genetics Molecular Sequence Data Protein Structure, Secondary Restriction Mapping

Chemicals

Halorhodopsins Bacteriorhodopsins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Blanck A

Max-Planck Institut für Biochemie, D-8033, Martinsried, FRG.

Oesterhelt D

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30 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1987-01-00

Pages

265-73

Language

English

Region

England

NLM ID

8208664

PMCID

PMC553387

Subset

External Links

PubMed source PMC Full Text View journal details

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The halo-opsin gene. II. Sequence, primary structure of halorhodopsin and comparison with bacteriorhodopsin.

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