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PMID: 15983060 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.

The Journal of cell biology ·Vol. 170 ·No. 1 ·2005-07-04 ·Pages 141-51

Smith A, Carrasco YR, Stanley P, Kieffer N, Batista FD, Hogg N

Abstract

Cells such as fibroblasts and endothelial cells migrate through the coordinated responses of discrete integrin-containing focal adhesions and complexes. In contrast, little is known about the organization of integrins on the highly motile T lymphocyte. We have investigated the distribution, activity, and cytoskeletal linkage of the integrin lymphocyte function associated antigen-1 (LFA-1) on human T lymphocytes migrating on endothelial cells and on ligand intercellular adhesion molecule-1 (ICAM-1). The pattern of total LFA-1 varies from low expression in the lamellipodia to high expression in the uropod. However, high affinity, clustered LFA-1 is restricted to a mid-cell zone that remains stable over time and over a range of ICAM-1 densities. Talin is essential for the stability and formation of the LFA-1 zone. Disruption of the talin-integrin link leads to loss of zone integrity and a substantial decrease in speed of migration on ICAM-1. This adhesive structure, which differs from the previously described integrin-containing attachments displayed by many other cell types, we have termed the "focal zone."

MeSH Terms
Cell Adhesion/physiology Cell Communication/immunology Cell Line Cell Membrane/immunology,metabolism Cells, Cultured Chemotaxis, Leukocyte/physiology Focal Adhesions/immunology,metabolism Humans Integrins/metabolism Intercellular Adhesion Molecule-1/metabolism Lymphocyte Function-Associated Antigen-1/metabolism Pseudopodia/immunology,metabolism T-Lymphocytes/immunology,metabolism Talin/metabolism Time Factors
Chemicals
Integrins Lymphocyte Function-Associated Antigen-1 Talin Intercellular Adhesion Molecule-1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Smith Andrew
Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, England, UK.
Carrasco Yolanda R
Stanley Paula
Kieffer Nelly
Batista Facundo D
Hogg Nancy
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200412032
Published
2005-07-04
Epub
2005-00-27
Pages
141-51
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171377
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200412032 PMC Full Text View journal details
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