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PMID: 15990873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The MAP kinase substrate MKS1 is a regulator of plant defense responses.

The EMBO journal ·Vol. 24 ·No. 14 ·2005-07-20 ·Pages 2579-89

Andreasson E, Jenkins T, Brodersen P, Thorgrimsen S, Petersen NH, Zhu S, Qiu JL, Micheelsen P, Rocher A, Petersen M, Newman MA, Bjørn Nielsen H, Hirt H, Somssich I, Mattsson O, Mundy J

Abstract

Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.

MeSH Terms
Amino Acid Motifs Amino Acid Sequence Arabidopsis Proteins/metabolism,physiology Gene Expression Profiling Immunity, Innate Immunohistochemistry Mitogen-Activated Protein Kinases/metabolism Molecular Sequence Data Nuclear Proteins Phosphoproteins/metabolism Phosphorylation Plants/enzymology,immunology Saccharomyces cerevisiae/metabolism Substrate Specificity Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins MKS1 protein, Arabidopsis Nuclear Proteins Phosphoproteins AtMPK4 protein, Arabidopsis Mitogen-Activated Protein Kinases
Authors & Affiliations
16 authors, click to expand affiliations / ORCID
Andreasson Erik
Molecular Biology Institute, University of Copenhagen, Copenhagen, Denmark.
Jenkins Thomas
Brodersen Peter
Thorgrimsen Stephan
Petersen Nikolaj H T
Zhu Shijiang
Qiu Jin-Long
Micheelsen Pernille
Rocher Anne
Petersen Morten
Newman Mari-Anne
Bjørn Nielsen Henrik
Hirt Heribert
Somssich Imre
Mattsson Ole
Mundy John
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7600737
Published
2005-07-20
Epub
2005-00-30
Pages
2579-89
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1176463
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7600737 PMC Full Text View journal details
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